Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays

Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The...

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Autores principales: Jadhao, Samadhan J., Anderson, Larry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120902/
https://www.ncbi.nlm.nih.gov/pubmed/27464686
http://dx.doi.org/10.1007/978-1-4939-3687-8_4
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author Jadhao, Samadhan J.
Anderson, Larry J.
author_facet Jadhao, Samadhan J.
Anderson, Larry J.
author_sort Jadhao, Samadhan J.
collection PubMed
description Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma.
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spelling pubmed-71209022020-04-06 Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays Jadhao, Samadhan J. Anderson, Larry J. Human Respiratory Syncytial Virus Article Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma. 2016-07-28 /pmc/articles/PMC7120902/ /pubmed/27464686 http://dx.doi.org/10.1007/978-1-4939-3687-8_4 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Jadhao, Samadhan J.
Anderson, Larry J.
Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
title Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
title_full Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
title_fullStr Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
title_full_unstemmed Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
title_short Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
title_sort detection of rsv antibodies in human plasma by enzyme immunoassays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120902/
https://www.ncbi.nlm.nih.gov/pubmed/27464686
http://dx.doi.org/10.1007/978-1-4939-3687-8_4
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