RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot

Of the many primer combinations that we have investigated for the detection of avian coronaviruses, two have worked better than any of the others: they worked with the largest number of strains/samples of a given coronavirus and the most species of avian coronavirus, and they also produced the most...

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Autores principales: Culver, Francesca Anne, Britton, Paul, Cavanagh, Dave
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120991/
https://www.ncbi.nlm.nih.gov/pubmed/19057865
http://dx.doi.org/10.1007/978-1-59745-181-9_4
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author Culver, Francesca Anne
Britton, Paul
Cavanagh, Dave
author_facet Culver, Francesca Anne
Britton, Paul
Cavanagh, Dave
author_sort Culver, Francesca Anne
collection PubMed
description Of the many primer combinations that we have investigated for the detection of avian coronaviruses, two have worked better than any of the others: they worked with the largest number of strains/samples of a given coronavirus and the most species of avian coronavirus, and they also produced the most sensitive detection tests. The primer combinations were: oligonucleotide pair 2Bp/4Bm, which is in a region of gene 1 that is moderately conserved among all species of coronavirus (1); and UTR11-/UTR41+, which are in a highly conserved part of the 3′ untranslated region of avian coronaviruses related to infectious bronchitis virus (2). The gene 1 primer pair enabled the detection of a new coronavirus in a green-cheeked Amazon parrot (Amazon viridigenalis Cassin). In this chapter we describe the use of these oligonucleotides in a one-step (single-tube) RT-PCR, and describe the procedure that we used to extract RNA from turkey feces.
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spelling pubmed-71209912020-04-06 RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot Culver, Francesca Anne Britton, Paul Cavanagh, Dave SARS- and Other Coronaviruses Article Of the many primer combinations that we have investigated for the detection of avian coronaviruses, two have worked better than any of the others: they worked with the largest number of strains/samples of a given coronavirus and the most species of avian coronavirus, and they also produced the most sensitive detection tests. The primer combinations were: oligonucleotide pair 2Bp/4Bm, which is in a region of gene 1 that is moderately conserved among all species of coronavirus (1); and UTR11-/UTR41+, which are in a highly conserved part of the 3′ untranslated region of avian coronaviruses related to infectious bronchitis virus (2). The gene 1 primer pair enabled the detection of a new coronavirus in a green-cheeked Amazon parrot (Amazon viridigenalis Cassin). In this chapter we describe the use of these oligonucleotides in a one-step (single-tube) RT-PCR, and describe the procedure that we used to extract RNA from turkey feces. 2007-11-28 /pmc/articles/PMC7120991/ /pubmed/19057865 http://dx.doi.org/10.1007/978-1-59745-181-9_4 Text en © Humana Press, a part of Springer Science+Business Media, LLC 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Culver, Francesca Anne
Britton, Paul
Cavanagh, Dave
RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
title RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
title_full RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
title_fullStr RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
title_full_unstemmed RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
title_short RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
title_sort rt-pcr detection of avian coronaviruses of galliform birds (chicken, turkey, pheasant) and in a parrot
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120991/
https://www.ncbi.nlm.nih.gov/pubmed/19057865
http://dx.doi.org/10.1007/978-1-59745-181-9_4
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