The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration

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Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation...

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Detalles Bibliográficos
Autores principales: Jones, Brenda V., Hennion, Ruth M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121057/
https://www.ncbi.nlm.nih.gov/pubmed/19057860
http://dx.doi.org/10.1007/978-1-59745-181-9_9
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author Jones, Brenda V.
Hennion, Ruth M.
author_facet Jones, Brenda V.
Hennion, Ruth M.
author_sort Jones, Brenda V.
collection PubMed
description Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
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spelling pubmed-71210572020-04-06 The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration Jones, Brenda V. Hennion, Ruth M. SARS- and Other Coronaviruses Article Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described. 2007-11-28 /pmc/articles/PMC7121057/ /pubmed/19057860 http://dx.doi.org/10.1007/978-1-59745-181-9_9 Text en © Humana Press, a part of Springer Science+Business Media, LLC 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Jones, Brenda V.
Hennion, Ruth M.
The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_full The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_fullStr The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_full_unstemmed The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_short The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_sort preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121057/
https://www.ncbi.nlm.nih.gov/pubmed/19057860
http://dx.doi.org/10.1007/978-1-59745-181-9_9
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