Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction

Bovine coronavirus (BCoV) is an economically significant cause of enteric and respiratory diseases in cattle throughout the world. BCoV is a known cause of neonatal calf diarrhea, winter dysentery in adult cattle, and respiratory disorders in cattle of all ages. In this chapter, we describe a simple...

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Autores principales: Alfieri, Amauri Alcindo, Alfieri, Alice Fernandes, Takiuchi, Elisabete
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121124/
http://dx.doi.org/10.1007/978-1-4939-3414-0_9
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author Alfieri, Amauri Alcindo
Alfieri, Alice Fernandes
Takiuchi, Elisabete
author_facet Alfieri, Amauri Alcindo
Alfieri, Alice Fernandes
Takiuchi, Elisabete
author_sort Alfieri, Amauri Alcindo
collection PubMed
description Bovine coronavirus (BCoV) is an economically significant cause of enteric and respiratory diseases in cattle throughout the world. BCoV is a known cause of neonatal calf diarrhea, winter dysentery in adult cattle, and respiratory disorders in cattle of all ages. In this chapter, we describe a simple and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle.
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spelling pubmed-71211242020-04-06 Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction Alfieri, Amauri Alcindo Alfieri, Alice Fernandes Takiuchi, Elisabete Animal Coronaviruses Article Bovine coronavirus (BCoV) is an economically significant cause of enteric and respiratory diseases in cattle throughout the world. BCoV is a known cause of neonatal calf diarrhea, winter dysentery in adult cattle, and respiratory disorders in cattle of all ages. In this chapter, we describe a simple and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle. 2015-09-10 /pmc/articles/PMC7121124/ http://dx.doi.org/10.1007/978-1-4939-3414-0_9 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Alfieri, Amauri Alcindo
Alfieri, Alice Fernandes
Takiuchi, Elisabete
Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction
title Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction
title_full Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction
title_fullStr Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction
title_full_unstemmed Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction
title_short Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction
title_sort detection of bovine coronavirus by conventional reverse transcription polymerase chain reaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121124/
http://dx.doi.org/10.1007/978-1-4939-3414-0_9
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