Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function. In contrast, oxidative dama...

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Autores principales: Nees, Gernot, Kaufmann, Andreas, Bauer, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121164/
https://www.ncbi.nlm.nih.gov/pubmed/24957224
http://dx.doi.org/10.1007/978-1-4939-0882-0_1
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author Nees, Gernot
Kaufmann, Andreas
Bauer, Stefan
author_facet Nees, Gernot
Kaufmann, Andreas
Bauer, Stefan
author_sort Nees, Gernot
collection PubMed
description Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function. In contrast, oxidative damage can lead to random modifications in rRNA and messenger RNA (mRNA) that strongly impair functionality. RNA modifications have also been implicated in avoiding self-RNA recognition by the immune system or immune evasion by pathogens. Here, we describe the detection of RNA modifications by HPLC analysis and competitive ELISA.
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spelling pubmed-71211642020-04-06 Detection of RNA Modifications by HPLC Analysis and Competitive ELISA Nees, Gernot Kaufmann, Andreas Bauer, Stefan Innate DNA and RNA Recognition Article Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function. In contrast, oxidative damage can lead to random modifications in rRNA and messenger RNA (mRNA) that strongly impair functionality. RNA modifications have also been implicated in avoiding self-RNA recognition by the immune system or immune evasion by pathogens. Here, we describe the detection of RNA modifications by HPLC analysis and competitive ELISA. 2014-03-20 /pmc/articles/PMC7121164/ /pubmed/24957224 http://dx.doi.org/10.1007/978-1-4939-0882-0_1 Text en © Springer Science+Business Media, New York 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Nees, Gernot
Kaufmann, Andreas
Bauer, Stefan
Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
title Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
title_full Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
title_fullStr Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
title_full_unstemmed Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
title_short Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
title_sort detection of rna modifications by hplc analysis and competitive elisa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121164/
https://www.ncbi.nlm.nih.gov/pubmed/24957224
http://dx.doi.org/10.1007/978-1-4939-0882-0_1
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