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Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function. In contrast, oxidative dama...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121164/ https://www.ncbi.nlm.nih.gov/pubmed/24957224 http://dx.doi.org/10.1007/978-1-4939-0882-0_1 |
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author | Nees, Gernot Kaufmann, Andreas Bauer, Stefan |
author_facet | Nees, Gernot Kaufmann, Andreas Bauer, Stefan |
author_sort | Nees, Gernot |
collection | PubMed |
description | Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function. In contrast, oxidative damage can lead to random modifications in rRNA and messenger RNA (mRNA) that strongly impair functionality. RNA modifications have also been implicated in avoiding self-RNA recognition by the immune system or immune evasion by pathogens. Here, we describe the detection of RNA modifications by HPLC analysis and competitive ELISA. |
format | Online Article Text |
id | pubmed-7121164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71211642020-04-06 Detection of RNA Modifications by HPLC Analysis and Competitive ELISA Nees, Gernot Kaufmann, Andreas Bauer, Stefan Innate DNA and RNA Recognition Article Over 100 different RNA modifications exist that are introduced posttranscriptionally by enzymes at specific nucleotide positions. Ribosomal RNA (rRNA) and transfer RNA (tRNA) exhibit the most and diverse modifications that presumably optimize their structure and function. In contrast, oxidative damage can lead to random modifications in rRNA and messenger RNA (mRNA) that strongly impair functionality. RNA modifications have also been implicated in avoiding self-RNA recognition by the immune system or immune evasion by pathogens. Here, we describe the detection of RNA modifications by HPLC analysis and competitive ELISA. 2014-03-20 /pmc/articles/PMC7121164/ /pubmed/24957224 http://dx.doi.org/10.1007/978-1-4939-0882-0_1 Text en © Springer Science+Business Media, New York 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Nees, Gernot Kaufmann, Andreas Bauer, Stefan Detection of RNA Modifications by HPLC Analysis and Competitive ELISA |
title | Detection of RNA Modifications by HPLC Analysis and Competitive ELISA |
title_full | Detection of RNA Modifications by HPLC Analysis and Competitive ELISA |
title_fullStr | Detection of RNA Modifications by HPLC Analysis and Competitive ELISA |
title_full_unstemmed | Detection of RNA Modifications by HPLC Analysis and Competitive ELISA |
title_short | Detection of RNA Modifications by HPLC Analysis and Competitive ELISA |
title_sort | detection of rna modifications by hplc analysis and competitive elisa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121164/ https://www.ncbi.nlm.nih.gov/pubmed/24957224 http://dx.doi.org/10.1007/978-1-4939-0882-0_1 |
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