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Sequencing DNA Amplified Directly from a Bacterial Colony

A few hundred bacterial cells obtained by touching a bacterial colony with a sterile toothpick can be used directly in a polymerase chain reaction (PCR) amplification procedure to identify and orient a plasmid insert (1,2). By combining this procedure with one in which asymmetrically amplified DNA i...

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Detalles Bibliográficos
Autores principales:	Hofmann, Martin A., Brian, David A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	1993
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121285/ https://www.ncbi.nlm.nih.gov/pubmed/21400278 http://dx.doi.org/10.1385/0-89603-244-2:205

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