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Design of Gene Constructs for Transgenic Maize

The first step of any maize transformation project is to select gene expression elements that will make up an effective construct. When designing a gene construct, one must have a full understanding of the different expression elements that are currently available and of the strategies that have bee...

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Detalles Bibliográficos
Autor principal: Liu, Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121396/
https://www.ncbi.nlm.nih.gov/pubmed/19378011
http://dx.doi.org/10.1007/978-1-59745-494-0_1
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author Liu, Dong
author_facet Liu, Dong
author_sort Liu, Dong
collection PubMed
description The first step of any maize transformation project is to select gene expression elements that will make up an effective construct. When designing a gene construct, one must have a full understanding of the different expression elements that are currently available and of the strategies that have been successfully used to overcome obstacles in past. In this chapter, we discuss several major classes of expression elements that have been used for maize transformation, including promoters, introns, and untranslated regions. We also discuss several strategies for further improving transgene expression levels, such as optimization of codon usage, removal of deleterious sequences, addition of signal sequences for subcellular protein targeting, and use of elements to reduce position effects. We hope that this chapter can serve as a general guideline to help researchers, especially beginners in the field, to design a gene construct that will have the maximum potential for gene expression.
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spelling pubmed-71213962020-04-06 Design of Gene Constructs for Transgenic Maize Liu, Dong Transgenic Maize Article The first step of any maize transformation project is to select gene expression elements that will make up an effective construct. When designing a gene construct, one must have a full understanding of the different expression elements that are currently available and of the strategies that have been successfully used to overcome obstacles in past. In this chapter, we discuss several major classes of expression elements that have been used for maize transformation, including promoters, introns, and untranslated regions. We also discuss several strategies for further improving transgene expression levels, such as optimization of codon usage, removal of deleterious sequences, addition of signal sequences for subcellular protein targeting, and use of elements to reduce position effects. We hope that this chapter can serve as a general guideline to help researchers, especially beginners in the field, to design a gene construct that will have the maximum potential for gene expression. 2009 /pmc/articles/PMC7121396/ /pubmed/19378011 http://dx.doi.org/10.1007/978-1-59745-494-0_1 Text en © Humana Press, a part of Springer Science+Business Media, LLC 2009 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Liu, Dong
Design of Gene Constructs for Transgenic Maize
title Design of Gene Constructs for Transgenic Maize
title_full Design of Gene Constructs for Transgenic Maize
title_fullStr Design of Gene Constructs for Transgenic Maize
title_full_unstemmed Design of Gene Constructs for Transgenic Maize
title_short Design of Gene Constructs for Transgenic Maize
title_sort design of gene constructs for transgenic maize
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121396/
https://www.ncbi.nlm.nih.gov/pubmed/19378011
http://dx.doi.org/10.1007/978-1-59745-494-0_1
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