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Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization
For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforwar...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121525/ https://www.ncbi.nlm.nih.gov/pubmed/19057878 http://dx.doi.org/10.1007/978-1-59745-181-9_13 |
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author | Piotrowski, Yvonne Ponnusamy, Rajesh Glaser, Stephanie Daabach, Anniken Moll, Ralf Hilgenfeld, Rolf |
author_facet | Piotrowski, Yvonne Ponnusamy, Rajesh Glaser, Stephanie Daabach, Anniken Moll, Ralf Hilgenfeld, Rolf |
author_sort | Piotrowski, Yvonne |
collection | PubMed |
description | For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities. |
format | Online Article Text |
id | pubmed-7121525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71215252020-04-06 Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization Piotrowski, Yvonne Ponnusamy, Rajesh Glaser, Stephanie Daabach, Anniken Moll, Ralf Hilgenfeld, Rolf SARS- and Other Coronaviruses Article For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities. 2007-11-28 /pmc/articles/PMC7121525/ /pubmed/19057878 http://dx.doi.org/10.1007/978-1-59745-181-9_13 Text en © Humana Press, a part of Springer Science+Business Media, LLC 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Piotrowski, Yvonne Ponnusamy, Rajesh Glaser, Stephanie Daabach, Anniken Moll, Ralf Hilgenfeld, Rolf Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization |
title | Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization |
title_full | Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization |
title_fullStr | Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization |
title_full_unstemmed | Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization |
title_short | Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization |
title_sort | production of coronavirus nonstructural proteins in soluble form for crystallization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121525/ https://www.ncbi.nlm.nih.gov/pubmed/19057878 http://dx.doi.org/10.1007/978-1-59745-181-9_13 |
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