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Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles

Biosensor for the detection of virus was developed by utilizing plasmonic peak shift phenomenon of the gold nanoparticles and viral infection mechanism of hemagglutinin on virus and sialic acid on animal cells. The plasmonic peak of the colloidal gold nanoparticles changes with the aggregation of th...

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Detalles Bibliográficos
Autores principales: Lee, Changwon, Wang, Peng, Gaston, Marsha A., Weiss, Alison A., Zhang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121675/
https://www.ncbi.nlm.nih.gov/pubmed/28281252
http://dx.doi.org/10.1007/978-1-4939-6848-0_7
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author Lee, Changwon
Wang, Peng
Gaston, Marsha A.
Weiss, Alison A.
Zhang, Peng
author_facet Lee, Changwon
Wang, Peng
Gaston, Marsha A.
Weiss, Alison A.
Zhang, Peng
author_sort Lee, Changwon
collection PubMed
description Biosensor for the detection of virus was developed by utilizing plasmonic peak shift phenomenon of the gold nanoparticles and viral infection mechanism of hemagglutinin on virus and sialic acid on animal cells. The plasmonic peak of the colloidal gold nanoparticles changes with the aggregation of the particles due to the plasmonic interaction between nearby particles and the color of the colloidal nanoparticle solution changes from wine red to purple. Sialic acid reduced and stabilized colloidal gold nanoparticle aggregation is induced by the addition of viral particles in the solution due to the hemagglutinin-sialic acid interaction. In this work, sialic acid reduced and stabilized gold nanoparticles (d = 20.1 ± 1.8 nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutinin-sialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B virus species. The detection limit of the virus dilution (hemagglutinination assay titer, 512) was shown to be 0.156 vol% and the upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles.
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spelling pubmed-71216752020-04-06 Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles Lee, Changwon Wang, Peng Gaston, Marsha A. Weiss, Alison A. Zhang, Peng Biosensors and Biodetection Article Biosensor for the detection of virus was developed by utilizing plasmonic peak shift phenomenon of the gold nanoparticles and viral infection mechanism of hemagglutinin on virus and sialic acid on animal cells. The plasmonic peak of the colloidal gold nanoparticles changes with the aggregation of the particles due to the plasmonic interaction between nearby particles and the color of the colloidal nanoparticle solution changes from wine red to purple. Sialic acid reduced and stabilized colloidal gold nanoparticle aggregation is induced by the addition of viral particles in the solution due to the hemagglutinin-sialic acid interaction. In this work, sialic acid reduced and stabilized gold nanoparticles (d = 20.1 ± 1.8 nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutinin-sialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B virus species. The detection limit of the virus dilution (hemagglutinination assay titer, 512) was shown to be 0.156 vol% and the upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles. 2017-03-10 /pmc/articles/PMC7121675/ /pubmed/28281252 http://dx.doi.org/10.1007/978-1-4939-6848-0_7 Text en © Springer Science+Business Media LLC 2017 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Lee, Changwon
Wang, Peng
Gaston, Marsha A.
Weiss, Alison A.
Zhang, Peng
Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles
title Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles
title_full Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles
title_fullStr Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles
title_full_unstemmed Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles
title_short Plasmonics-Based Detection of Virus Using Sialic Acid Functionalized Gold Nanoparticles
title_sort plasmonics-based detection of virus using sialic acid functionalized gold nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121675/
https://www.ncbi.nlm.nih.gov/pubmed/28281252
http://dx.doi.org/10.1007/978-1-4939-6848-0_7
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