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Construction of Simple and Efficient siRNA Validation Systems for Screening and Identification of Effective RNAi-Targeted Sequences from Mammalian Genes
RNA interference (RNAi) is an evolutionarily conserved mechanism of gene silencing induced by double-stranded RNAs (dsRNAs). Among the widely used dsRNAs, small interfering RNAs (siRNAs) and short hairpin RNAs have evolved as extremely powerful and the most popular gene silencing reagents. The key c...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121774/ https://www.ncbi.nlm.nih.gov/pubmed/24233788 http://dx.doi.org/10.1007/978-1-62703-721-1_15 |