Studying Evolutionary Adaptation of MERS-CoV

Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which...

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Detalles Bibliográficos
Autores principales: Letko, Michael, Munster, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121928/
https://www.ncbi.nlm.nih.gov/pubmed/31883083
http://dx.doi.org/10.1007/978-1-0716-0211-9_1
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author Letko, Michael
Munster, Vincent
author_facet Letko, Michael
Munster, Vincent
author_sort Letko, Michael
collection PubMed
description Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018.
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spelling pubmed-71219282020-04-06 Studying Evolutionary Adaptation of MERS-CoV Letko, Michael Munster, Vincent MERS Coronavirus Article Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730–1737, 2018. 2019-09-14 /pmc/articles/PMC7121928/ /pubmed/31883083 http://dx.doi.org/10.1007/978-1-0716-0211-9_1 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Letko, Michael
Munster, Vincent
Studying Evolutionary Adaptation of MERS-CoV
title Studying Evolutionary Adaptation of MERS-CoV
title_full Studying Evolutionary Adaptation of MERS-CoV
title_fullStr Studying Evolutionary Adaptation of MERS-CoV
title_full_unstemmed Studying Evolutionary Adaptation of MERS-CoV
title_short Studying Evolutionary Adaptation of MERS-CoV
title_sort studying evolutionary adaptation of mers-cov
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121928/
https://www.ncbi.nlm.nih.gov/pubmed/31883083
http://dx.doi.org/10.1007/978-1-0716-0211-9_1
work_keys_str_mv AT letkomichael studyingevolutionaryadaptationofmerscov
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