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Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle
Quantitative proteomics, based on stable isotope labeling by amino acids in cell culture (SILAC), can be used to identify host proteins involved in the intracellular interplay with pathogens. This method allows identification of proteins subject to degradation or upregulation in response to intracel...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121999/ https://www.ncbi.nlm.nih.gov/pubmed/25253246 http://dx.doi.org/10.1007/978-1-4939-1625-2_2 |
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author | Kaloyanova, Dora Vogels, Mijke van Balkom, Bas W. M. Helms, J. Bernd |
author_facet | Kaloyanova, Dora Vogels, Mijke van Balkom, Bas W. M. Helms, J. Bernd |
author_sort | Kaloyanova, Dora |
collection | PubMed |
description | Quantitative proteomics, based on stable isotope labeling by amino acids in cell culture (SILAC), can be used to identify host proteins involved in the intracellular interplay with pathogens. This method allows identification of proteins subject to degradation or upregulation in response to intracellular infection. It can also be used to study intracellular dynamics (trafficking) of proteins in response to the infection. Here, we describe the analysis of changes in protein profiles determined in Golgi-enriched fractions isolated from cells that were either mock-infected or infected with Salmonella typhimurium. Using the SILAC approach we were able to identify 105 proteins in Golgi-enriched fractions that were significantly changed in their abundance as a result of Salmonella infection. |
format | Online Article Text |
id | pubmed-7121999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71219992020-04-06 Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle Kaloyanova, Dora Vogels, Mijke van Balkom, Bas W. M. Helms, J. Bernd Salmonella Article Quantitative proteomics, based on stable isotope labeling by amino acids in cell culture (SILAC), can be used to identify host proteins involved in the intracellular interplay with pathogens. This method allows identification of proteins subject to degradation or upregulation in response to intracellular infection. It can also be used to study intracellular dynamics (trafficking) of proteins in response to the infection. Here, we describe the analysis of changes in protein profiles determined in Golgi-enriched fractions isolated from cells that were either mock-infected or infected with Salmonella typhimurium. Using the SILAC approach we were able to identify 105 proteins in Golgi-enriched fractions that were significantly changed in their abundance as a result of Salmonella infection. 2014-09-08 /pmc/articles/PMC7121999/ /pubmed/25253246 http://dx.doi.org/10.1007/978-1-4939-1625-2_2 Text en © Springer Science+Business Media New York 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Kaloyanova, Dora Vogels, Mijke van Balkom, Bas W. M. Helms, J. Bernd Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle |
title | Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle |
title_full | Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle |
title_fullStr | Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle |
title_full_unstemmed | Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle |
title_short | Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle |
title_sort | quantitative proteomic identification of host factors involved in the salmonella typhimurium infection cycle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121999/ https://www.ncbi.nlm.nih.gov/pubmed/25253246 http://dx.doi.org/10.1007/978-1-4939-1625-2_2 |
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