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Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope
Transmission electron microscopy (TEM) is an invaluable technique used for imaging the ultrastructure of samples and it is particularly useful when determining virus–host interactions at a cellular level. The environment inside a TEM is not favorable for biological material (high vacuum and high ene...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122084/ https://www.ncbi.nlm.nih.gov/pubmed/25720488 http://dx.doi.org/10.1007/978-1-4939-2438-7_23 |