Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen

Turkey coronavirus (TCoV) infection continues to threaten turkey industry. Because specific treatment and effective vaccination program are not available, rapid and cost-effective detection of antibodies to TCoV infection is an important control measure to monitor the disease status in the fields. T...

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Autores principales: Loa, Chien Chang, Abdelwahab, Mohamed, Chen, Yi-Ning, Hsieh, Ming-Kun, Wu, Ching Ching, Lin, Tsang Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122138/
http://dx.doi.org/10.1007/978-1-4939-3414-0_5
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author Loa, Chien Chang
Abdelwahab, Mohamed
Chen, Yi-Ning
Hsieh, Ming-Kun
Wu, Ching Ching
Lin, Tsang Long
author_facet Loa, Chien Chang
Abdelwahab, Mohamed
Chen, Yi-Ning
Hsieh, Ming-Kun
Wu, Ching Ching
Lin, Tsang Long
author_sort Loa, Chien Chang
collection PubMed
description Turkey coronavirus (TCoV) infection continues to threaten turkey industry. Because specific treatment and effective vaccination program are not available, rapid and cost-effective detection of antibodies to TCoV infection is an important control measure to monitor the disease status in the fields. Two antibody-capture enzyme-linked immunosorbent assay (ELISA) procedures for detection of antibodies to TCoV are outlined in this chapter. One ELISA method uses chicken infectious bronchitis coronavirus (IBV) as the coating antigen based on antigenic cross-reactivity between TCoV and IBV. The other method relies on a recombinant TCoV nucleocapsid protein. Both methods are useful for serological diagnosis of TCoV infection in the turkey flocks.
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spelling pubmed-71221382020-04-06 Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen Loa, Chien Chang Abdelwahab, Mohamed Chen, Yi-Ning Hsieh, Ming-Kun Wu, Ching Ching Lin, Tsang Long Animal Coronaviruses Article Turkey coronavirus (TCoV) infection continues to threaten turkey industry. Because specific treatment and effective vaccination program are not available, rapid and cost-effective detection of antibodies to TCoV infection is an important control measure to monitor the disease status in the fields. Two antibody-capture enzyme-linked immunosorbent assay (ELISA) procedures for detection of antibodies to TCoV are outlined in this chapter. One ELISA method uses chicken infectious bronchitis coronavirus (IBV) as the coating antigen based on antigenic cross-reactivity between TCoV and IBV. The other method relies on a recombinant TCoV nucleocapsid protein. Both methods are useful for serological diagnosis of TCoV infection in the turkey flocks. 2015-09-10 /pmc/articles/PMC7122138/ http://dx.doi.org/10.1007/978-1-4939-3414-0_5 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Loa, Chien Chang
Abdelwahab, Mohamed
Chen, Yi-Ning
Hsieh, Ming-Kun
Wu, Ching Ching
Lin, Tsang Long
Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen
title Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen
title_full Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen
title_fullStr Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen
title_full_unstemmed Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen
title_short Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen
title_sort antibody-capture enzyme-linked immunosorbent assay for detection of antibody to turkey coronavirus using infectious bronchitis virus or recombinant nucleocapsid protein as coating antigen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122138/
http://dx.doi.org/10.1007/978-1-4939-3414-0_5
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