Transgene Design

Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and genera...

Descripción completa

Detalles Bibliográficos
Autores principales: van de Sluis, Bart, Voncken, Jan Willem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122210/
https://www.ncbi.nlm.nih.gov/pubmed/21080276
http://dx.doi.org/10.1007/978-1-60761-974-1_6
_version_ 1783515368111734784
author van de Sluis, Bart
Voncken, Jan Willem
author_facet van de Sluis, Bart
Voncken, Jan Willem
author_sort van de Sluis, Bart
collection PubMed
description Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed. Methods for DNA purification for microinjection are also provided in this chapter.
format Online
Article
Text
id pubmed-7122210
institution National Center for Biotechnology Information
language English
publishDate 2010
record_format MEDLINE/PubMed
spelling pubmed-71222102020-04-06 Transgene Design van de Sluis, Bart Voncken, Jan Willem Transgenic Mouse Methods and Protocols Article Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed. Methods for DNA purification for microinjection are also provided in this chapter. 2010-10-04 /pmc/articles/PMC7122210/ /pubmed/21080276 http://dx.doi.org/10.1007/978-1-60761-974-1_6 Text en © Springer Science+Business Media, LLC 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
van de Sluis, Bart
Voncken, Jan Willem
Transgene Design
title Transgene Design
title_full Transgene Design
title_fullStr Transgene Design
title_full_unstemmed Transgene Design
title_short Transgene Design
title_sort transgene design
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122210/
https://www.ncbi.nlm.nih.gov/pubmed/21080276
http://dx.doi.org/10.1007/978-1-60761-974-1_6
work_keys_str_mv AT vandesluisbart transgenedesign
AT vonckenjanwillem transgenedesign

Ejemplares similares