Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA

Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of li...

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Autores principales: Al-amri, Sawsan S., Hashem, Anwar M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122216/
https://www.ncbi.nlm.nih.gov/pubmed/31883093
http://dx.doi.org/10.1007/978-1-0716-0211-9_11
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author Al-amri, Sawsan S.
Hashem, Anwar M.
author_facet Al-amri, Sawsan S.
Hashem, Anwar M.
author_sort Al-amri, Sawsan S.
collection PubMed
description Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1–725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies.
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spelling pubmed-71222162020-04-06 Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA Al-amri, Sawsan S. Hashem, Anwar M. MERS Coronavirus Article Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1–725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies. 2019-09-14 /pmc/articles/PMC7122216/ /pubmed/31883093 http://dx.doi.org/10.1007/978-1-0716-0211-9_11 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Al-amri, Sawsan S.
Hashem, Anwar M.
Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA
title Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA
title_full Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA
title_fullStr Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA
title_full_unstemmed Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA
title_short Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA
title_sort qualitative and quantitative determination of mers-cov s1-specific antibodies using elisa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122216/
https://www.ncbi.nlm.nih.gov/pubmed/31883093
http://dx.doi.org/10.1007/978-1-0716-0211-9_11
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