The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics

Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatm...

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Autores principales: Sari, Duygu, Gupta, Kapil, Raj, Deepak Balaji Thimiri Govinda, Aubert, Alice, Drncová, Petra, Garzoni, Frederic, Fitzgerald, Daniel, Berger, Imre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122245/
https://www.ncbi.nlm.nih.gov/pubmed/27165327
http://dx.doi.org/10.1007/978-3-319-27216-0_13
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author Sari, Duygu
Gupta, Kapil
Raj, Deepak Balaji Thimiri Govinda
Aubert, Alice
Drncová, Petra
Garzoni, Frederic
Fitzgerald, Daniel
Berger, Imre
author_facet Sari, Duygu
Gupta, Kapil
Raj, Deepak Balaji Thimiri Govinda
Aubert, Alice
Drncová, Petra
Garzoni, Frederic
Fitzgerald, Daniel
Berger, Imre
author_sort Sari, Duygu
collection PubMed
description Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatment of human diseases. Recombinant production by the baculovirus/insect cell expression system is particularly useful for expressing proteins of eukaryotic origin and their complexes. MultiBac, an advanced baculovirus/insect cell system, has been widely adopted in the last decade to produce multiprotein complexes with many subunits that were hitherto inaccessible, for academic and industrial research and development. The MultiBac system, its development and numerous applications are presented. Future opportunities for utilizing MultiBac to catalyze discovery are outlined.
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spelling pubmed-71222452020-04-06 The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics Sari, Duygu Gupta, Kapil Raj, Deepak Balaji Thimiri Govinda Aubert, Alice Drncová, Petra Garzoni, Frederic Fitzgerald, Daniel Berger, Imre Advanced Technologies for Protein Complex Production and Characterization Article Multiprotein complexes regulate most if not all cellular functions. Elucidating the structure and function of these complex cellular machines is essential for understanding biology. Moreover, multiprotein complexes by themselves constitute powerful reagents as biologics for the prevention and treatment of human diseases. Recombinant production by the baculovirus/insect cell expression system is particularly useful for expressing proteins of eukaryotic origin and their complexes. MultiBac, an advanced baculovirus/insect cell system, has been widely adopted in the last decade to produce multiprotein complexes with many subunits that were hitherto inaccessible, for academic and industrial research and development. The MultiBac system, its development and numerous applications are presented. Future opportunities for utilizing MultiBac to catalyze discovery are outlined. 2015-12-12 /pmc/articles/PMC7122245/ /pubmed/27165327 http://dx.doi.org/10.1007/978-3-319-27216-0_13 Text en © Springer International Publishing Switzerland 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Sari, Duygu
Gupta, Kapil
Raj, Deepak Balaji Thimiri Govinda
Aubert, Alice
Drncová, Petra
Garzoni, Frederic
Fitzgerald, Daniel
Berger, Imre
The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
title The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
title_full The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
title_fullStr The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
title_full_unstemmed The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
title_short The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
title_sort multibac baculovirus/insect cell expression vector system for producing complex protein biologics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122245/
https://www.ncbi.nlm.nih.gov/pubmed/27165327
http://dx.doi.org/10.1007/978-3-319-27216-0_13
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