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Expression and Purification of Recombinant Proteins in Escherichia coli with a His(6) or Dual His(6)-MBP Tag
Rapid advances in bioengineering and biotechnology over the past three decades have greatly facilitated the production of recombinant proteins in Escherichia coli. Affinity-based methods that employ protein or peptide based tags for protein purification have been instrumental in this progress. Yet i...
Autores principales: | Raran-Kurussi, Sreejith, Waugh, David S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122414/ https://www.ncbi.nlm.nih.gov/pubmed/28573567 http://dx.doi.org/10.1007/978-1-4939-7000-1_1 |
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