The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration

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Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation...

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Autor principal: Hennion, Ruth M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122565/
https://www.ncbi.nlm.nih.gov/pubmed/25720470
http://dx.doi.org/10.1007/978-1-4939-2438-7_5
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author Hennion, Ruth M.
author_facet Hennion, Ruth M.
author_sort Hennion, Ruth M.
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description Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
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spelling pubmed-71225652020-04-06 The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration Hennion, Ruth M. Coronaviruses Article Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described. 2014-12-18 /pmc/articles/PMC7122565/ /pubmed/25720470 http://dx.doi.org/10.1007/978-1-4939-2438-7_5 Text en © Springer Science+Business Media New York 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Hennion, Ruth M.
The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_full The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_fullStr The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_full_unstemmed The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_short The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
title_sort preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122565/
https://www.ncbi.nlm.nih.gov/pubmed/25720470
http://dx.doi.org/10.1007/978-1-4939-2438-7_5
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