The Preparation of Chicken Kidney Cell Cultures for Virus Propagation

Chicken kidney (CK) cell cultures have historically proved useful for the assay of a number of viruses including coronaviruses. A technique for the preparation of such cell cultures, using a combination of manual and trypsin disaggregation of kidneys dissected from 2- to 3-week-old birds is describe...

Descripción completa

Detalles Bibliográficos
Autores principales: Hennion, Ruth M., Hill, Gillian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122669/
https://www.ncbi.nlm.nih.gov/pubmed/25720471
http://dx.doi.org/10.1007/978-1-4939-2438-7_6
_version_ 1783515469087506432
author Hennion, Ruth M.
Hill, Gillian
author_facet Hennion, Ruth M.
Hill, Gillian
author_sort Hennion, Ruth M.
collection PubMed
description Chicken kidney (CK) cell cultures have historically proved useful for the assay of a number of viruses including coronaviruses. A technique for the preparation of such cell cultures, using a combination of manual and trypsin disaggregation of kidneys dissected from 2- to 3-week-old birds is described. This technique routinely gives high cell yield together with high viability and the resultant adherent primary cultures can be used for virus growth and plaque formation.
format Online
Article
Text
id pubmed-7122669
institution National Center for Biotechnology Information
language English
publishDate 2014
record_format MEDLINE/PubMed
spelling pubmed-71226692020-04-06 The Preparation of Chicken Kidney Cell Cultures for Virus Propagation Hennion, Ruth M. Hill, Gillian Coronaviruses Article Chicken kidney (CK) cell cultures have historically proved useful for the assay of a number of viruses including coronaviruses. A technique for the preparation of such cell cultures, using a combination of manual and trypsin disaggregation of kidneys dissected from 2- to 3-week-old birds is described. This technique routinely gives high cell yield together with high viability and the resultant adherent primary cultures can be used for virus growth and plaque formation. 2014-12-18 /pmc/articles/PMC7122669/ /pubmed/25720471 http://dx.doi.org/10.1007/978-1-4939-2438-7_6 Text en © Springer Science+Business Media New York 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Hennion, Ruth M.
Hill, Gillian
The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
title The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
title_full The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
title_fullStr The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
title_full_unstemmed The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
title_short The Preparation of Chicken Kidney Cell Cultures for Virus Propagation
title_sort preparation of chicken kidney cell cultures for virus propagation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122669/
https://www.ncbi.nlm.nih.gov/pubmed/25720471
http://dx.doi.org/10.1007/978-1-4939-2438-7_6
work_keys_str_mv AT hennionruthm thepreparationofchickenkidneycellculturesforviruspropagation
AT hillgillian thepreparationofchickenkidneycellculturesforviruspropagation
AT hennionruthm preparationofchickenkidneycellculturesforviruspropagation
AT hillgillian preparationofchickenkidneycellculturesforviruspropagation

Ejemplares similares