PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene

Turkey coronaviral enteritis caused by turkey coronavirus (TCoV) continues to infect turkey flocks, resulting in significant economic loss. Determining and understanding genetic relationships among different TCoV isolates or strains is important for controlling the disease. Using two-step RT-PCR ass...

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Autores principales: Chen, Yi-Ning, Akin, Aydemir, Loa, Chien Chang, Ababneh, Mustafa, Cao, Jianzhong, Chen, Wan-Jung, Wu, Ching Ching, Lin, Tsang Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122697/
http://dx.doi.org/10.1007/978-1-4939-3414-0_14
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author Chen, Yi-Ning
Akin, Aydemir
Loa, Chien Chang
Ababneh, Mustafa
Cao, Jianzhong
Chen, Wan-Jung
Wu, Ching Ching
Lin, Tsang Long
author_facet Chen, Yi-Ning
Akin, Aydemir
Loa, Chien Chang
Ababneh, Mustafa
Cao, Jianzhong
Chen, Wan-Jung
Wu, Ching Ching
Lin, Tsang Long
author_sort Chen, Yi-Ning
collection PubMed
description Turkey coronaviral enteritis caused by turkey coronavirus (TCoV) continues to infect turkey flocks, resulting in significant economic loss. Determining and understanding genetic relationships among different TCoV isolates or strains is important for controlling the disease. Using two-step RT-PCR assays that amplify the full length of TCoV spike (S) gene, TCoV isolates can be sequenced, analyzed, and genotyped. Described in this chapter is the protocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks.
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spelling pubmed-71226972020-04-06 PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene Chen, Yi-Ning Akin, Aydemir Loa, Chien Chang Ababneh, Mustafa Cao, Jianzhong Chen, Wan-Jung Wu, Ching Ching Lin, Tsang Long Animal Coronaviruses Article Turkey coronaviral enteritis caused by turkey coronavirus (TCoV) continues to infect turkey flocks, resulting in significant economic loss. Determining and understanding genetic relationships among different TCoV isolates or strains is important for controlling the disease. Using two-step RT-PCR assays that amplify the full length of TCoV spike (S) gene, TCoV isolates can be sequenced, analyzed, and genotyped. Described in this chapter is the protocol on PCR amplification and sequencing analysis of full-length TCoV S gene. Such protocol is useful in molecular epidemiology for establishing an effective strategy to control the transmission of TCoV among turkey flocks. 2015-09-10 /pmc/articles/PMC7122697/ http://dx.doi.org/10.1007/978-1-4939-3414-0_14 Text en © Springer Science+Business Media New York 2016 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Chen, Yi-Ning
Akin, Aydemir
Loa, Chien Chang
Ababneh, Mustafa
Cao, Jianzhong
Chen, Wan-Jung
Wu, Ching Ching
Lin, Tsang Long
PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
title PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
title_full PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
title_fullStr PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
title_full_unstemmed PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
title_short PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene
title_sort pcr amplification and sequencing analysis of full-length turkey coronavirus spike gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122697/
http://dx.doi.org/10.1007/978-1-4939-3414-0_14
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