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Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women

BACKGROUND AND OBJECTIVE: In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). METHOD...

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Autores principales: Vesentini, Giovana, Barbosa, Angélica M. P., Damasceno, Débora C., Marini, Gabriela, Piculo, Fernanda, Matheus, Selma M. M., Hallur, Raghavendra L. S., Nunes, Sthefanie K., Catinelli, Bruna B., Magalhães, Claudia G., Costa, Roberto, Abbade, Joelcio F., Corrente, José E., Calderon, Iracema M. P., Rudge, Marilza V. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122752/
https://www.ncbi.nlm.nih.gov/pubmed/32243473
http://dx.doi.org/10.1371/journal.pone.0231096
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author Vesentini, Giovana
Barbosa, Angélica M. P.
Damasceno, Débora C.
Marini, Gabriela
Piculo, Fernanda
Matheus, Selma M. M.
Hallur, Raghavendra L. S.
Nunes, Sthefanie K.
Catinelli, Bruna B.
Magalhães, Claudia G.
Costa, Roberto
Abbade, Joelcio F.
Corrente, José E.
Calderon, Iracema M. P.
Rudge, Marilza V. C.
author_facet Vesentini, Giovana
Barbosa, Angélica M. P.
Damasceno, Débora C.
Marini, Gabriela
Piculo, Fernanda
Matheus, Selma M. M.
Hallur, Raghavendra L. S.
Nunes, Sthefanie K.
Catinelli, Bruna B.
Magalhães, Claudia G.
Costa, Roberto
Abbade, Joelcio F.
Corrente, José E.
Calderon, Iracema M. P.
Rudge, Marilza V. C.
author_sort Vesentini, Giovana
collection PubMed
description BACKGROUND AND OBJECTIVE: In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). METHOD: Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. RESULTS: No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. CONCLUSION: Our results indicated that pregnancy- associated diabetes- induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM.
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spelling pubmed-71227522020-04-09 Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women Vesentini, Giovana Barbosa, Angélica M. P. Damasceno, Débora C. Marini, Gabriela Piculo, Fernanda Matheus, Selma M. M. Hallur, Raghavendra L. S. Nunes, Sthefanie K. Catinelli, Bruna B. Magalhães, Claudia G. Costa, Roberto Abbade, Joelcio F. Corrente, José E. Calderon, Iracema M. P. Rudge, Marilza V. C. PLoS One Research Article BACKGROUND AND OBJECTIVE: In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). METHOD: Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. RESULTS: No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. CONCLUSION: Our results indicated that pregnancy- associated diabetes- induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM. Public Library of Science 2020-04-03 /pmc/articles/PMC7122752/ /pubmed/32243473 http://dx.doi.org/10.1371/journal.pone.0231096 Text en © 2020 Vesentini et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vesentini, Giovana
Barbosa, Angélica M. P.
Damasceno, Débora C.
Marini, Gabriela
Piculo, Fernanda
Matheus, Selma M. M.
Hallur, Raghavendra L. S.
Nunes, Sthefanie K.
Catinelli, Bruna B.
Magalhães, Claudia G.
Costa, Roberto
Abbade, Joelcio F.
Corrente, José E.
Calderon, Iracema M. P.
Rudge, Marilza V. C.
Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women
title Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women
title_full Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women
title_fullStr Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women
title_full_unstemmed Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women
title_short Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women
title_sort alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: a comparative study of the rat model and women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122752/
https://www.ncbi.nlm.nih.gov/pubmed/32243473
http://dx.doi.org/10.1371/journal.pone.0231096
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