Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera

Pseudotyped viral particle production has been used extensively and broadly for many viruses to evaluate levels of neutralizing antibodies, viral entry inhibitors and vaccine immunogenicity. This assay is extremely safe and useful alternative to live virus-based assay without the need for high conta...

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Detalles Bibliográficos
Autores principales: Almasaud, Abdulrahman, Alharbi, Naif Khalaf, Hashem, Anwar M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123069/
https://www.ncbi.nlm.nih.gov/pubmed/31883092
http://dx.doi.org/10.1007/978-1-0716-0211-9_10
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author Almasaud, Abdulrahman
Alharbi, Naif Khalaf
Hashem, Anwar M.
author_facet Almasaud, Abdulrahman
Alharbi, Naif Khalaf
Hashem, Anwar M.
author_sort Almasaud, Abdulrahman
collection PubMed
description Pseudotyped viral particle production has been used extensively and broadly for many viruses to evaluate levels of neutralizing antibodies, viral entry inhibitors and vaccine immunogenicity. This assay is extremely safe and useful alternative to live virus-based assay without the need for high containment facilities. In this chapter, we describe the generation of MERS-CoV pseudotyped viral particles (MERSpp) expressing full-length spike protein using second-generation lentiviral packaging system. This platform is optimized to generate high titer of MERSpp and to test sera from different mammalian species.
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spelling pubmed-71230692020-04-06 Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera Almasaud, Abdulrahman Alharbi, Naif Khalaf Hashem, Anwar M. MERS Coronavirus Article Pseudotyped viral particle production has been used extensively and broadly for many viruses to evaluate levels of neutralizing antibodies, viral entry inhibitors and vaccine immunogenicity. This assay is extremely safe and useful alternative to live virus-based assay without the need for high containment facilities. In this chapter, we describe the generation of MERS-CoV pseudotyped viral particles (MERSpp) expressing full-length spike protein using second-generation lentiviral packaging system. This platform is optimized to generate high titer of MERSpp and to test sera from different mammalian species. 2019-09-14 /pmc/articles/PMC7123069/ /pubmed/31883092 http://dx.doi.org/10.1007/978-1-0716-0211-9_10 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Almasaud, Abdulrahman
Alharbi, Naif Khalaf
Hashem, Anwar M.
Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
title Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
title_full Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
title_fullStr Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
title_full_unstemmed Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
title_short Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
title_sort generation of mers-cov pseudotyped viral particles for the evaluation of neutralizing antibodies in mammalian sera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123069/
https://www.ncbi.nlm.nih.gov/pubmed/31883092
http://dx.doi.org/10.1007/978-1-0716-0211-9_10
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AT alharbinaifkhalaf generationofmerscovpseudotypedviralparticlesfortheevaluationofneutralizingantibodiesinmammaliansera
AT hashemanwarm generationofmerscovpseudotypedviralparticlesfortheevaluationofneutralizingantibodiesinmammaliansera

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