Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein

Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs...

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Detalles Bibliográficos
Autores principales: Zhou, Haixia, Zhang, Shuyuan, Wang, Xinquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123399/
https://www.ncbi.nlm.nih.gov/pubmed/31883086
http://dx.doi.org/10.1007/978-1-0716-0211-9_4
Descripción
Sumario:Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs at the atomic level. In this chapter, we describe the purification, crystallization, and structure determination of the MERS-CoV RBD.

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