Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein

Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs...

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Autores principales: Zhou, Haixia, Zhang, Shuyuan, Wang, Xinquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123399/
https://www.ncbi.nlm.nih.gov/pubmed/31883086
http://dx.doi.org/10.1007/978-1-0716-0211-9_4
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author Zhou, Haixia
Zhang, Shuyuan
Wang, Xinquan
author_facet Zhou, Haixia
Zhang, Shuyuan
Wang, Xinquan
author_sort Zhou, Haixia
collection PubMed
description Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs at the atomic level. In this chapter, we describe the purification, crystallization, and structure determination of the MERS-CoV RBD.
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spelling pubmed-71233992020-04-06 Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein Zhou, Haixia Zhang, Shuyuan Wang, Xinquan MERS Coronavirus Article Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs at the atomic level. In this chapter, we describe the purification, crystallization, and structure determination of the MERS-CoV RBD. 2019-09-14 /pmc/articles/PMC7123399/ /pubmed/31883086 http://dx.doi.org/10.1007/978-1-0716-0211-9_4 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Zhou, Haixia
Zhang, Shuyuan
Wang, Xinquan
Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein
title Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein
title_full Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein
title_fullStr Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein
title_full_unstemmed Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein
title_short Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein
title_sort crystallization and structural determination of the receptor-binding domain of mers-cov spike glycoprotein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123399/
https://www.ncbi.nlm.nih.gov/pubmed/31883086
http://dx.doi.org/10.1007/978-1-0716-0211-9_4
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AT zhangshuyuan crystallizationandstructuraldeterminationofthereceptorbindingdomainofmerscovspikeglycoprotein
AT wangxinquan crystallizationandstructuraldeterminationofthereceptorbindingdomainofmerscovspikeglycoprotein

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