Quantification of Interferon Signaling in Avian Cells

Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantifica...

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Detalles Bibliográficos
Autores principales: Kint, Joeri, Forlenza, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123703/
https://www.ncbi.nlm.nih.gov/pubmed/25720486
http://dx.doi.org/10.1007/978-1-4939-2438-7_21
Descripción
Sumario:Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay.

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