Quantification of Interferon Signaling in Avian Cells

Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantifica...

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Detalles Bibliográficos
Autores principales: Kint, Joeri, Forlenza, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123703/
https://www.ncbi.nlm.nih.gov/pubmed/25720486
http://dx.doi.org/10.1007/978-1-4939-2438-7_21
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author Kint, Joeri
Forlenza, Maria
author_facet Kint, Joeri
Forlenza, Maria
author_sort Kint, Joeri
collection PubMed
description Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay.
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spelling pubmed-71237032020-04-06 Quantification of Interferon Signaling in Avian Cells Kint, Joeri Forlenza, Maria Coronaviruses Article Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay. 2014-12-18 /pmc/articles/PMC7123703/ /pubmed/25720486 http://dx.doi.org/10.1007/978-1-4939-2438-7_21 Text en © Springer Science+Business Media New York 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Kint, Joeri
Forlenza, Maria
Quantification of Interferon Signaling in Avian Cells
title Quantification of Interferon Signaling in Avian Cells
title_full Quantification of Interferon Signaling in Avian Cells
title_fullStr Quantification of Interferon Signaling in Avian Cells
title_full_unstemmed Quantification of Interferon Signaling in Avian Cells
title_short Quantification of Interferon Signaling in Avian Cells
title_sort quantification of interferon signaling in avian cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123703/
https://www.ncbi.nlm.nih.gov/pubmed/25720486
http://dx.doi.org/10.1007/978-1-4939-2438-7_21
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