Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems

In order to illuminate the antigenicity of porcine transmissible gastroenteritis virus (TGEV) spike protein B and C antigen sites, the truncated spike gene including B and C antigen sites of Chinese isolate TH-98 was expressed respectively in E.coli, baculovirus and pichia pastoris expression system...

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Autores principales: Yu, Tian-fei, Shao, Shu-li, Xu, Xing-jun, Lv, Jian-wei, Li, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123857/
http://dx.doi.org/10.1007/978-3-642-27537-1_7
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author Yu, Tian-fei
Shao, Shu-li
Xu, Xing-jun
Lv, Jian-wei
Li, Ming
author_facet Yu, Tian-fei
Shao, Shu-li
Xu, Xing-jun
Lv, Jian-wei
Li, Ming
author_sort Yu, Tian-fei
collection PubMed
description In order to illuminate the antigenicity of porcine transmissible gastroenteritis virus (TGEV) spike protein B and C antigen sites, the truncated spike gene including B and C antigen sites of Chinese isolate TH-98 was expressed respectively in E.coli, baculovirus and pichia pastoris expression systems. Dot enzyme-linked immunosorbent assays (Dot-ELISA) based on these three recombinant proteins were developed preliminarily. Ten sera obtained correspondingly from ten piglets two months old which showed up clinical symptom were used for examination. The study indicates that the assays are rapid, reliable and sensitive and it has the potential for use as serological methods for TGEV diagnosis.
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spelling pubmed-71238572020-04-06 Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems Yu, Tian-fei Shao, Shu-li Xu, Xing-jun Lv, Jian-wei Li, Ming Information Technology and Agricultural Engineering Article In order to illuminate the antigenicity of porcine transmissible gastroenteritis virus (TGEV) spike protein B and C antigen sites, the truncated spike gene including B and C antigen sites of Chinese isolate TH-98 was expressed respectively in E.coli, baculovirus and pichia pastoris expression systems. Dot enzyme-linked immunosorbent assays (Dot-ELISA) based on these three recombinant proteins were developed preliminarily. Ten sera obtained correspondingly from ten piglets two months old which showed up clinical symptom were used for examination. The study indicates that the assays are rapid, reliable and sensitive and it has the potential for use as serological methods for TGEV diagnosis. 2012 /pmc/articles/PMC7123857/ http://dx.doi.org/10.1007/978-3-642-27537-1_7 Text en © Springer-Verlag GmbH Berlin Heidelberg 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Yu, Tian-fei
Shao, Shu-li
Xu, Xing-jun
Lv, Jian-wei
Li, Ming
Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems
title Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems
title_full Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems
title_fullStr Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems
title_full_unstemmed Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems
title_short Express Transmissible Gastroenteritis Virus Spike Gene B and C Antigen Sites in Multiple Expression Systems
title_sort express transmissible gastroenteritis virus spike gene b and c antigen sites in multiple expression systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7123857/
http://dx.doi.org/10.1007/978-3-642-27537-1_7
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