Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures

The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluore...

Descripción completa

Detalles Bibliográficos
Autores principales: Jonsdottir, Hulda R., Dijkman, Ronald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124022/
https://www.ncbi.nlm.nih.gov/pubmed/25720473
http://dx.doi.org/10.1007/978-1-4939-2438-7_8
_version_ 1783515764772306944
author Jonsdottir, Hulda R.
Dijkman, Ronald
author_facet Jonsdottir, Hulda R.
Dijkman, Ronald
author_sort Jonsdottir, Hulda R.
collection PubMed
description The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication.
format Online
Article
Text
id pubmed-7124022
institution National Center for Biotechnology Information
language English
publishDate 2014
record_format MEDLINE/PubMed
spelling pubmed-71240222020-04-06 Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures Jonsdottir, Hulda R. Dijkman, Ronald Coronaviruses Article The human airway serves as the entry point of human respiratory viruses, including human coronaviruses. In this chapter we outline the methods by which we establish fully differentiated airway epithelium and its use for human coronavirus propagation. Additionally, we outline methods for immunofluorescence staining of these cultures for virus detection, characterization of cell tropism, and how to perform antiviral assays and quantify viral replication. 2014-12-18 /pmc/articles/PMC7124022/ /pubmed/25720473 http://dx.doi.org/10.1007/978-1-4939-2438-7_8 Text en © Springer Science+Business Media New York 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Jonsdottir, Hulda R.
Dijkman, Ronald
Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
title Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
title_full Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
title_fullStr Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
title_full_unstemmed Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
title_short Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures
title_sort characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124022/
https://www.ncbi.nlm.nih.gov/pubmed/25720473
http://dx.doi.org/10.1007/978-1-4939-2438-7_8
work_keys_str_mv AT jonsdottirhuldar characterizationofhumancoronavirusesonwelldifferentiatedhumanairwayepithelialcellcultures
AT dijkmanronald characterizationofhumancoronavirusesonwelldifferentiatedhumanairwayepithelialcellcultures

Ejemplares similares