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Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus
In this study, we isolated and characterized mesenchymal stromal cells (MSCs) from the lungs of 1- to 2-week-old chickens. Microscopically, the cultured cells showed fibroblast-like morphology. Phenotypically these cells expressed CD44, CD90, CD105 and the transcription factor PouV, which has been s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124449/ https://www.ncbi.nlm.nih.gov/pubmed/20026347 http://dx.doi.org/10.1016/j.dci.2009.12.008 |
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author | Khatri, Mahesh O’Brien, Timothy D. Goyal, Sagar M. Sharma, Jagdev M. |
author_facet | Khatri, Mahesh O’Brien, Timothy D. Goyal, Sagar M. Sharma, Jagdev M. |
author_sort | Khatri, Mahesh |
collection | PubMed |
description | In this study, we isolated and characterized mesenchymal stromal cells (MSCs) from the lungs of 1- to 2-week-old chickens. Microscopically, the cultured cells showed fibroblast-like morphology. Phenotypically these cells expressed CD44, CD90, CD105 and the transcription factor PouV, which has been shown to be critical for stem cell self-renewal and pluripotency. The multipotency of chicken MSCs was demonstrated by their ability to undergo adipogenic and osteogenic differentiation. Like chicken bone marrow MSCs and mammalian MSCs, chicken lung MSCs had immunoregulatory activity and profoundly suppressed the proliferative capacity of T cells in response to a mitogenic stimulus. Next, we examined the susceptibility of these cells to H1N1 and H9N5 avian influenza (AI) viruses. The lung MSCs were shown to express known influenza virus alpha-2,3 and alpha-2,6 sialic acid receptors and to support replication of both the avian H1N1 and avian H9N5 influenza strains. Viral infection of MSCs resulted in cell lysis and cytokine and chemokine production. Further characterization of lung MSCs in chicken and other mammalian species may help in understanding the pathogenesis of infectious and non-infectious lung diseases and the mechanisms of lung injury repair. |
format | Online Article Text |
id | pubmed-7124449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71244492020-04-06 Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus Khatri, Mahesh O’Brien, Timothy D. Goyal, Sagar M. Sharma, Jagdev M. Dev Comp Immunol Article In this study, we isolated and characterized mesenchymal stromal cells (MSCs) from the lungs of 1- to 2-week-old chickens. Microscopically, the cultured cells showed fibroblast-like morphology. Phenotypically these cells expressed CD44, CD90, CD105 and the transcription factor PouV, which has been shown to be critical for stem cell self-renewal and pluripotency. The multipotency of chicken MSCs was demonstrated by their ability to undergo adipogenic and osteogenic differentiation. Like chicken bone marrow MSCs and mammalian MSCs, chicken lung MSCs had immunoregulatory activity and profoundly suppressed the proliferative capacity of T cells in response to a mitogenic stimulus. Next, we examined the susceptibility of these cells to H1N1 and H9N5 avian influenza (AI) viruses. The lung MSCs were shown to express known influenza virus alpha-2,3 and alpha-2,6 sialic acid receptors and to support replication of both the avian H1N1 and avian H9N5 influenza strains. Viral infection of MSCs resulted in cell lysis and cytokine and chemokine production. Further characterization of lung MSCs in chicken and other mammalian species may help in understanding the pathogenesis of infectious and non-infectious lung diseases and the mechanisms of lung injury repair. Elsevier Ltd. 2010-04 2009-12-28 /pmc/articles/PMC7124449/ /pubmed/20026347 http://dx.doi.org/10.1016/j.dci.2009.12.008 Text en Copyright © 2009 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Khatri, Mahesh O’Brien, Timothy D. Goyal, Sagar M. Sharma, Jagdev M. Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus |
title | Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus |
title_full | Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus |
title_fullStr | Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus |
title_full_unstemmed | Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus |
title_short | Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus |
title_sort | isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124449/ https://www.ncbi.nlm.nih.gov/pubmed/20026347 http://dx.doi.org/10.1016/j.dci.2009.12.008 |
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