An LC-QToF MS based method for untargeted metabolomics of human fecal samples

INTRODUCTION: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. OBJECTIVES: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. METHODS: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by me...

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Detalles Bibliográficos
Autores principales: Cheng, Ken, Brunius, Carl, Fristedt, Rikard, Landberg, Rikard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125068/
https://www.ncbi.nlm.nih.gov/pubmed/32246267
http://dx.doi.org/10.1007/s11306-020-01669-z
Descripción
Sumario:INTRODUCTION: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. OBJECTIVES: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. METHODS: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. RESULTS: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. CONCLUSION: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11306-020-01669-z) contains supplementary material, which is available to authorized users.

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