An LC-QToF MS based method for untargeted metabolomics of human fecal samples

INTRODUCTION: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. OBJECTIVES: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. METHODS: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by me...

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Autores principales: Cheng, Ken, Brunius, Carl, Fristedt, Rikard, Landberg, Rikard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125068/
https://www.ncbi.nlm.nih.gov/pubmed/32246267
http://dx.doi.org/10.1007/s11306-020-01669-z
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author Cheng, Ken
Brunius, Carl
Fristedt, Rikard
Landberg, Rikard
author_facet Cheng, Ken
Brunius, Carl
Fristedt, Rikard
Landberg, Rikard
author_sort Cheng, Ken
collection PubMed
description INTRODUCTION: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. OBJECTIVES: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. METHODS: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. RESULTS: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. CONCLUSION: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11306-020-01669-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-71250682020-04-06 An LC-QToF MS based method for untargeted metabolomics of human fecal samples Cheng, Ken Brunius, Carl Fristedt, Rikard Landberg, Rikard Metabolomics Short Communication INTRODUCTION: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. OBJECTIVES: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. METHODS: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. RESULTS: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. CONCLUSION: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11306-020-01669-z) contains supplementary material, which is available to authorized users. Springer US 2020-04-03 2020 /pmc/articles/PMC7125068/ /pubmed/32246267 http://dx.doi.org/10.1007/s11306-020-01669-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Short Communication
Cheng, Ken
Brunius, Carl
Fristedt, Rikard
Landberg, Rikard
An LC-QToF MS based method for untargeted metabolomics of human fecal samples
title An LC-QToF MS based method for untargeted metabolomics of human fecal samples
title_full An LC-QToF MS based method for untargeted metabolomics of human fecal samples
title_fullStr An LC-QToF MS based method for untargeted metabolomics of human fecal samples
title_full_unstemmed An LC-QToF MS based method for untargeted metabolomics of human fecal samples
title_short An LC-QToF MS based method for untargeted metabolomics of human fecal samples
title_sort lc-qtof ms based method for untargeted metabolomics of human fecal samples
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125068/
https://www.ncbi.nlm.nih.gov/pubmed/32246267
http://dx.doi.org/10.1007/s11306-020-01669-z
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