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First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA

The corn earworm, Helicoverpa zea, is a major target pest of the insecticidal Vip3Aa protein used in pyramided transgenic Bt corn and cotton with Cry1 and Cry2 proteins in the U.S. The widespread resistance to Cry1 and Cry2 proteins in H. zea will challenge the long-term efficacy of Vip3Aa technolog...

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Autores principales: Yang, Fei, González, José C. Santiago, Little, Nathan, Reisig, Dominic, Payne, Gregory, Dos Santos, Rafael Ferreira, Jurat-Fuentes, Juan Luis, Kurtz, Ryan, Kerns, David L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125131/
https://www.ncbi.nlm.nih.gov/pubmed/32246037
http://dx.doi.org/10.1038/s41598-020-62748-8
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author Yang, Fei
González, José C. Santiago
Little, Nathan
Reisig, Dominic
Payne, Gregory
Dos Santos, Rafael Ferreira
Jurat-Fuentes, Juan Luis
Kurtz, Ryan
Kerns, David L.
author_facet Yang, Fei
González, José C. Santiago
Little, Nathan
Reisig, Dominic
Payne, Gregory
Dos Santos, Rafael Ferreira
Jurat-Fuentes, Juan Luis
Kurtz, Ryan
Kerns, David L.
author_sort Yang, Fei
collection PubMed
description The corn earworm, Helicoverpa zea, is a major target pest of the insecticidal Vip3Aa protein used in pyramided transgenic Bt corn and cotton with Cry1 and Cry2 proteins in the U.S. The widespread resistance to Cry1 and Cry2 proteins in H. zea will challenge the long-term efficacy of Vip3Aa technology. Determining the frequency of resistant alleles to Vip3Aa in field populations of H. zea is critically important for resistance management. Here, we provided the first F(2) screen study to estimate the resistance allele frequency for Vip3Aa in H. zea populations in Texas, U.S. In 2019, 128 H. zea neonates per isofamily for a total of 114 F(2) families were screened with a diagnostic concentration of 3.0 μg/cm(2) of Vip3Aa39 protein in diet-overlay bioassays. The F(2) screen detected two families carrying a major Vip3Aa resistance allele. The estimated frequency of major resistance alleles against Vip3Aa39 in H. zea in Texas from this study was 0.0065 with a 95% CI of 0.0014–0.0157. A Vip3Aa-resistant strain (RR) derived from the F(2) screen showed a high level of resistance to Vip3Aa39 protein, with a resistance ratio of >588.0-fold relative to a susceptible population (SS) based on diet-overlay bioassays. We provide the first documentation of a major resistance allele conferring high levels of Vip3Aa resistance in a field-derived strain of H. zea in the U.S. Data generated from this study contribute to development of management strategies for the sustainable use of the Vip3Aa technology to control H. zea in the U.S.
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spelling pubmed-71251312020-04-08 First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA Yang, Fei González, José C. Santiago Little, Nathan Reisig, Dominic Payne, Gregory Dos Santos, Rafael Ferreira Jurat-Fuentes, Juan Luis Kurtz, Ryan Kerns, David L. Sci Rep Article The corn earworm, Helicoverpa zea, is a major target pest of the insecticidal Vip3Aa protein used in pyramided transgenic Bt corn and cotton with Cry1 and Cry2 proteins in the U.S. The widespread resistance to Cry1 and Cry2 proteins in H. zea will challenge the long-term efficacy of Vip3Aa technology. Determining the frequency of resistant alleles to Vip3Aa in field populations of H. zea is critically important for resistance management. Here, we provided the first F(2) screen study to estimate the resistance allele frequency for Vip3Aa in H. zea populations in Texas, U.S. In 2019, 128 H. zea neonates per isofamily for a total of 114 F(2) families were screened with a diagnostic concentration of 3.0 μg/cm(2) of Vip3Aa39 protein in diet-overlay bioassays. The F(2) screen detected two families carrying a major Vip3Aa resistance allele. The estimated frequency of major resistance alleles against Vip3Aa39 in H. zea in Texas from this study was 0.0065 with a 95% CI of 0.0014–0.0157. A Vip3Aa-resistant strain (RR) derived from the F(2) screen showed a high level of resistance to Vip3Aa39 protein, with a resistance ratio of >588.0-fold relative to a susceptible population (SS) based on diet-overlay bioassays. We provide the first documentation of a major resistance allele conferring high levels of Vip3Aa resistance in a field-derived strain of H. zea in the U.S. Data generated from this study contribute to development of management strategies for the sustainable use of the Vip3Aa technology to control H. zea in the U.S. Nature Publishing Group UK 2020-04-03 /pmc/articles/PMC7125131/ /pubmed/32246037 http://dx.doi.org/10.1038/s41598-020-62748-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Fei
González, José C. Santiago
Little, Nathan
Reisig, Dominic
Payne, Gregory
Dos Santos, Rafael Ferreira
Jurat-Fuentes, Juan Luis
Kurtz, Ryan
Kerns, David L.
First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA
title First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA
title_full First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA
title_fullStr First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA
title_full_unstemmed First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA
title_short First documentation of major Vip3Aa resistance alleles in field populations of Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) in Texas, USA
title_sort first documentation of major vip3aa resistance alleles in field populations of helicoverpa zea (boddie) (lepidoptera: noctuidae) in texas, usa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125131/
https://www.ncbi.nlm.nih.gov/pubmed/32246037
http://dx.doi.org/10.1038/s41598-020-62748-8
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