Cargando…
A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays
We report a group of complexes used in clinical immunoassays. The complexes include a PAMAM-conjugated goat anti-rabbit IgG and a QDs-conjugated goat anti-mouse IgG. When rabbit anti-antigen and mouse anti-antigen are added, the corresponding antigen will be detected. The experiment, using the compl...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125291/ https://www.ncbi.nlm.nih.gov/pubmed/32246298 http://dx.doi.org/10.1186/s11671-020-3291-5 |
_version_ | 1783515914557194240 |
---|---|
author | Liu, Yang Hou, Lu Guo, Qiong Zhang, Mingjin Shi, Wei |
author_facet | Liu, Yang Hou, Lu Guo, Qiong Zhang, Mingjin Shi, Wei |
author_sort | Liu, Yang |
collection | PubMed |
description | We report a group of complexes used in clinical immunoassays. The complexes include a PAMAM-conjugated goat anti-rabbit IgG and a QDs-conjugated goat anti-mouse IgG. When rabbit anti-antigen and mouse anti-antigen are added, the corresponding antigen will be detected. The experiment, using the complexes, is simple, convenient, short in time, and short in steps. It is also applicable to different experiment methods, like to be used with FCM (flow cytometry), ICC (immunocytochemistry), and IHC (immunohistochemistry) to detect many kinds of antigens. |
format | Online Article Text |
id | pubmed-7125291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-71252912020-04-08 A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays Liu, Yang Hou, Lu Guo, Qiong Zhang, Mingjin Shi, Wei Nanoscale Res Lett Nano Express We report a group of complexes used in clinical immunoassays. The complexes include a PAMAM-conjugated goat anti-rabbit IgG and a QDs-conjugated goat anti-mouse IgG. When rabbit anti-antigen and mouse anti-antigen are added, the corresponding antigen will be detected. The experiment, using the complexes, is simple, convenient, short in time, and short in steps. It is also applicable to different experiment methods, like to be used with FCM (flow cytometry), ICC (immunocytochemistry), and IHC (immunohistochemistry) to detect many kinds of antigens. Springer US 2020-04-03 /pmc/articles/PMC7125291/ /pubmed/32246298 http://dx.doi.org/10.1186/s11671-020-3291-5 Text en © The Author(s). 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Nano Express Liu, Yang Hou, Lu Guo, Qiong Zhang, Mingjin Shi, Wei A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays |
title | A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays |
title_full | A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays |
title_fullStr | A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays |
title_full_unstemmed | A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays |
title_short | A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays |
title_sort | group of complexes based on pamam and quantum dots used in clinical immunoassays |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125291/ https://www.ncbi.nlm.nih.gov/pubmed/32246298 http://dx.doi.org/10.1186/s11671-020-3291-5 |
work_keys_str_mv | AT liuyang agroupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT houlu agroupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT guoqiong agroupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT zhangmingjin agroupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT shiwei agroupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT liuyang groupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT houlu groupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT guoqiong groupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT zhangmingjin groupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays AT shiwei groupofcomplexesbasedonpamamandquantumdotsusedinclinicalimmunoassays |