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Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa
We describe herein a newly developed optical immunosensor for detection of antibodies directed against antigens of the Ebola virus strains Zaire and Sudan. We employed a photo immobilization methodology based on a photoactivatable electrogenerated poly(pyrrole-benzophenone) film deposited upon an in...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125681/ https://www.ncbi.nlm.nih.gov/pubmed/32288238 http://dx.doi.org/10.1016/j.snb.2006.07.005 |
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author | Petrosova, A. Konry, T. Cosnier, S. Trakht, I. Lutwama, J. Rwaguma, E. Chepurnov, A. Mühlberger, E. Lobel, L. Marks, R.S. |
author_facet | Petrosova, A. Konry, T. Cosnier, S. Trakht, I. Lutwama, J. Rwaguma, E. Chepurnov, A. Mühlberger, E. Lobel, L. Marks, R.S. |
author_sort | Petrosova, A. |
collection | PubMed |
description | We describe herein a newly developed optical immunosensor for detection of antibodies directed against antigens of the Ebola virus strains Zaire and Sudan. We employed a photo immobilization methodology based on a photoactivatable electrogenerated poly(pyrrole-benzophenone) film deposited upon an indium tin oxide (ITO) modified conductive surface fiber-optic. It was then linked to a biological receptor, Ebola virus antigen in this case, on the fiber tip through a light driven reaction. The photochemically modified optical fibers were tested as an immunosensor for detection of antibodies against Ebola virus, in animal and human sera, by use of a coupled chemiluminescent reaction. The immunosensor was tested for sensitivity, specificity, and compared to standard chemiluminescent ELISA under the same conditions. The analyte, anti-Ebola IgG, was detected at a low titer of 1:960,000 and 1:1,000,000 for subtypes Zaire and Sudan, respectively. While the same serum tested by ELISA was one order (24 times) less sensitive. |
format | Online Article Text |
id | pubmed-7125681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71256812020-04-08 Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa Petrosova, A. Konry, T. Cosnier, S. Trakht, I. Lutwama, J. Rwaguma, E. Chepurnov, A. Mühlberger, E. Lobel, L. Marks, R.S. Sens Actuators B Chem Article We describe herein a newly developed optical immunosensor for detection of antibodies directed against antigens of the Ebola virus strains Zaire and Sudan. We employed a photo immobilization methodology based on a photoactivatable electrogenerated poly(pyrrole-benzophenone) film deposited upon an indium tin oxide (ITO) modified conductive surface fiber-optic. It was then linked to a biological receptor, Ebola virus antigen in this case, on the fiber tip through a light driven reaction. The photochemically modified optical fibers were tested as an immunosensor for detection of antibodies against Ebola virus, in animal and human sera, by use of a coupled chemiluminescent reaction. The immunosensor was tested for sensitivity, specificity, and compared to standard chemiluminescent ELISA under the same conditions. The analyte, anti-Ebola IgG, was detected at a low titer of 1:960,000 and 1:1,000,000 for subtypes Zaire and Sudan, respectively. While the same serum tested by ELISA was one order (24 times) less sensitive. Elsevier B.V. 2007-03-26 2006-08-14 /pmc/articles/PMC7125681/ /pubmed/32288238 http://dx.doi.org/10.1016/j.snb.2006.07.005 Text en Copyright © 2006 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Petrosova, A. Konry, T. Cosnier, S. Trakht, I. Lutwama, J. Rwaguma, E. Chepurnov, A. Mühlberger, E. Lobel, L. Marks, R.S. Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa |
title | Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa |
title_full | Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa |
title_fullStr | Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa |
title_full_unstemmed | Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa |
title_short | Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa |
title_sort | development of a highly sensitive, field operable biosensor for serological studies of ebola virus in central africa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125681/ https://www.ncbi.nlm.nih.gov/pubmed/32288238 http://dx.doi.org/10.1016/j.snb.2006.07.005 |
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