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Biosynthesis of anti-HCV compounds using thermophilic microorganisms
This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthe...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Ltd.
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125738/ https://www.ncbi.nlm.nih.gov/pubmed/22959520 http://dx.doi.org/10.1016/j.bmcl.2012.08.045 |
| _version_ | 1783516007714783232 |
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| author | Rivero, Cintia W. De Benedetti, Eliana C. Sambeth, Jorge E. Lozano, Mario E. Trelles, Jorge A. |
| author_facet | Rivero, Cintia W. De Benedetti, Eliana C. Sambeth, Jorge E. Lozano, Mario E. Trelles, Jorge A. |
| author_sort | Rivero, Cintia W. |
| collection | PubMed |
| description | This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents. |
| format | Online Article Text |
| id | pubmed-7125738 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2012 |
| publisher | Elsevier Ltd. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-71257382020-04-08 Biosynthesis of anti-HCV compounds using thermophilic microorganisms Rivero, Cintia W. De Benedetti, Eliana C. Sambeth, Jorge E. Lozano, Mario E. Trelles, Jorge A. Bioorg Med Chem Lett Article This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents. Elsevier Ltd. 2012-10-01 2012-08-20 /pmc/articles/PMC7125738/ /pubmed/22959520 http://dx.doi.org/10.1016/j.bmcl.2012.08.045 Text en Copyright © 2012 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
| spellingShingle | Article Rivero, Cintia W. De Benedetti, Eliana C. Sambeth, Jorge E. Lozano, Mario E. Trelles, Jorge A. Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
| title | Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
| title_full | Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
| title_fullStr | Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
| title_full_unstemmed | Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
| title_short | Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
| title_sort | biosynthesis of anti-hcv compounds using thermophilic microorganisms |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125738/ https://www.ncbi.nlm.nih.gov/pubmed/22959520 http://dx.doi.org/10.1016/j.bmcl.2012.08.045 |
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