Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains

Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservati...

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Detalles Bibliográficos
Autores principales: Yuan, Shishan, Mickelson, Daniel, Murtaugh, Michael P., Faaberg, Kay S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2001
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125765/
https://www.ncbi.nlm.nih.gov/pubmed/11226578
http://dx.doi.org/10.1016/S0168-1702(00)00250-1
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author Yuan, Shishan
Mickelson, Daniel
Murtaugh, Michael P.
Faaberg, Kay S.
author_facet Yuan, Shishan
Mickelson, Daniel
Murtaugh, Michael P.
Faaberg, Kay S.
author_sort Yuan, Shishan
collection PubMed
description Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system.
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spelling pubmed-71257652020-04-08 Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains Yuan, Shishan Mickelson, Daniel Murtaugh, Michael P. Faaberg, Kay S. Virus Res Article Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system. Elsevier Science B.V. 2001-04 2001-02-20 /pmc/articles/PMC7125765/ /pubmed/11226578 http://dx.doi.org/10.1016/S0168-1702(00)00250-1 Text en Copyright © 2001 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Yuan, Shishan
Mickelson, Daniel
Murtaugh, Michael P.
Faaberg, Kay S.
Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
title Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
title_full Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
title_fullStr Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
title_full_unstemmed Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
title_short Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
title_sort complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125765/
https://www.ncbi.nlm.nih.gov/pubmed/11226578
http://dx.doi.org/10.1016/S0168-1702(00)00250-1
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