Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture

Recently, amiloride was shown to potently suppress Coxsackievirus B3 (CVB3) replication. In the current study, we investigated whether amiloride could also exhibit antiviral activity against foot-and-mouth disease virus (FMDV), which belongs to the same family (Picornaviridae) as CVB3. We found that...

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Detalles Bibliográficos
Autores principales: Gong, Mei-jiao, Chang, Yan-yan, Shao, Jun–jun, Li, Shi-fang, Zhang, Yong-guang, Chang, Hui-yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125800/
https://www.ncbi.nlm.nih.gov/pubmed/31326561
http://dx.doi.org/10.1016/j.micpath.2019.103638
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author Gong, Mei-jiao
Chang, Yan-yan
Shao, Jun–jun
Li, Shi-fang
Zhang, Yong-guang
Chang, Hui-yun
author_facet Gong, Mei-jiao
Chang, Yan-yan
Shao, Jun–jun
Li, Shi-fang
Zhang, Yong-guang
Chang, Hui-yun
author_sort Gong, Mei-jiao
collection PubMed
description Recently, amiloride was shown to potently suppress Coxsackievirus B3 (CVB3) replication. In the current study, we investigated whether amiloride could also exhibit antiviral activity against foot-and-mouth disease virus (FMDV), which belongs to the same family (Picornaviridae) as CVB3. We found that amiloride exerted antiviral activity in a dose-dependent manner against two strains of FMDV in IBRS-2 cells, with slight cytotoxicity at 1000 μM. Besides, amiloride did not inhibit the attachment and entry of FMDV in IBRS-2 cells, but prevented early viral replication. These data implied that amiloride could be a promising candidate for further research as a potential antiviral drug against FMDV infection.
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spelling pubmed-71258002020-04-08 Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture Gong, Mei-jiao Chang, Yan-yan Shao, Jun–jun Li, Shi-fang Zhang, Yong-guang Chang, Hui-yun Microb Pathog Article Recently, amiloride was shown to potently suppress Coxsackievirus B3 (CVB3) replication. In the current study, we investigated whether amiloride could also exhibit antiviral activity against foot-and-mouth disease virus (FMDV), which belongs to the same family (Picornaviridae) as CVB3. We found that amiloride exerted antiviral activity in a dose-dependent manner against two strains of FMDV in IBRS-2 cells, with slight cytotoxicity at 1000 μM. Besides, amiloride did not inhibit the attachment and entry of FMDV in IBRS-2 cells, but prevented early viral replication. These data implied that amiloride could be a promising candidate for further research as a potential antiviral drug against FMDV infection. Elsevier Ltd. 2019-10 2019-07-19 /pmc/articles/PMC7125800/ /pubmed/31326561 http://dx.doi.org/10.1016/j.micpath.2019.103638 Text en © 2019 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Gong, Mei-jiao
Chang, Yan-yan
Shao, Jun–jun
Li, Shi-fang
Zhang, Yong-guang
Chang, Hui-yun
Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
title Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
title_full Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
title_fullStr Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
title_full_unstemmed Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
title_short Antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
title_sort antiviral effect of amiloride on replication of foot and mouth disease virus in cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125800/
https://www.ncbi.nlm.nih.gov/pubmed/31326561
http://dx.doi.org/10.1016/j.micpath.2019.103638
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