Cargando…

Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs

ETHNOPHARMACOLOGICAL RELEVANCE: According to Traditional Chinese Medicine theory, influenza is categorized as a warm disease or Wen Bing. The Wen Bing formulas, such as Yin-Qiao-San and Sang-Ju-Yin, are still first-line herbal therapies in combating variant influenza virus. To continue our study on...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yufeng, Lyu, Chunming, Fong, Sophia Yui Kau, Wang, Qian, Li, Chenrui, Ho, Nicolas James, Chan, Kay Sheung, Yan, Xiaoyu, Zuo, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125811/
https://www.ncbi.nlm.nih.gov/pubmed/31325600
http://dx.doi.org/10.1016/j.jep.2019.112097
_version_ 1783516023898505216
author Zhang, Yufeng
Lyu, Chunming
Fong, Sophia Yui Kau
Wang, Qian
Li, Chenrui
Ho, Nicolas James
Chan, Kay Sheung
Yan, Xiaoyu
Zuo, Zhong
author_facet Zhang, Yufeng
Lyu, Chunming
Fong, Sophia Yui Kau
Wang, Qian
Li, Chenrui
Ho, Nicolas James
Chan, Kay Sheung
Yan, Xiaoyu
Zuo, Zhong
author_sort Zhang, Yufeng
collection PubMed
description ETHNOPHARMACOLOGICAL RELEVANCE: According to Traditional Chinese Medicine theory, influenza is categorized as a warm disease or Wen Bing. The Wen Bing formulas, such as Yin-Qiao-San and Sang-Ju-Yin, are still first-line herbal therapies in combating variant influenza virus. To continue our study on the pharmacokinetic and pharmacodynamic interactions between Wen Bing formulas and oseltamivir (OS), the first-line western drug for the treatment of influenza, further interactions between OS and the eight single herbs and their relevant marker components from Wen Bing formulas were investigated in the current study. AIM OF STUDY: To establish an in-vitro screening platform for investigation of the potential anti-influenza herbs/herbal components that may have pharmacokinetic and pharmacodynamic interactions with OS. MATERIALS AND METHODS: To screen potential inhibition on OS hydrolysis, 1 μg/mL of OS is incubated with herbs/herbal components in diluted rat plasma, microsomes and human recombinant carboxylesterase 1(hCE1) under optimized conditions. MDCK-WT and MDCK-MDR1 cell lines are utilized to identify potential modification on P-gp mediated transport of OS by herbs/herbal components. Caco-2 cells with and without Gly-Sar inhibition are performed to study the uptake of OS via PEPT1 transporters. Modification on OAT3 mediated transport is verified by the uptake of OS on HEK293-MOCK/HEK293-OAT3 cells. Anti-virus effects were evaluated using plaque reduction assay on H1N1 and H3N2 viruses. Potential pharmacokinetic and pharmacodynamic interaction between OS (30 mg/kg) and the selected herb, Radix Scutellariae (RS), at 300–600 mg/kg were carried out on rats. All samples are analyzed by an LC/MS/MS method for the contents of OS and OSA. A mechanistic PK model was developed to interpret the HDI between OS and RS in rats. RESULTS: Our developed platform was successfully applied to screen the eight herbal extracts and their ten marker components on metabolic inhibition of OS and modification of OS transport mediated by P-gp, OAT3 and PEPT1. Results from six in-vitro experiments were analyzed after converting raw data from each experiment to corresponding fold-change (FC) values, based on which Radix Scutellariae (RS) were selected to have the most HDI potential with OS. By analyzing the plasma and urine pharmacokinetic data after co-administration of OS with a standardized RS extract in rats using an integrated population pharmacokinetics model, it is suggested that RS could inhibit OS hydrolysis during absorption and increase the absorbed fraction of OS, which leads to the increased ratio of OS concentration versus that of OSA in both rat plasma and urine. Never the less, the anti-virus effects of 2.5 h post-dose rat plasma were not influenced by co-administration of OS with RS. CONCLUSION: A six-dimension in-vitro screening platform has been developed and successfully applied to find RS as a potential herb that would influence the co-administrated OS in rats. Although co-administered RS could inhibit OS hydrolysis during absorption and increase the absorbed fraction of OS, which lead to the increased ratio of OS concentration versus that of OSA in both rat plasma and urine, the anti-virus effect of OS was not influenced by co-administered RS.
format Online
Article
Text
id pubmed-7125811
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71258112020-04-08 Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs Zhang, Yufeng Lyu, Chunming Fong, Sophia Yui Kau Wang, Qian Li, Chenrui Ho, Nicolas James Chan, Kay Sheung Yan, Xiaoyu Zuo, Zhong J Ethnopharmacol Article ETHNOPHARMACOLOGICAL RELEVANCE: According to Traditional Chinese Medicine theory, influenza is categorized as a warm disease or Wen Bing. The Wen Bing formulas, such as Yin-Qiao-San and Sang-Ju-Yin, are still first-line herbal therapies in combating variant influenza virus. To continue our study on the pharmacokinetic and pharmacodynamic interactions between Wen Bing formulas and oseltamivir (OS), the first-line western drug for the treatment of influenza, further interactions between OS and the eight single herbs and their relevant marker components from Wen Bing formulas were investigated in the current study. AIM OF STUDY: To establish an in-vitro screening platform for investigation of the potential anti-influenza herbs/herbal components that may have pharmacokinetic and pharmacodynamic interactions with OS. MATERIALS AND METHODS: To screen potential inhibition on OS hydrolysis, 1 μg/mL of OS is incubated with herbs/herbal components in diluted rat plasma, microsomes and human recombinant carboxylesterase 1(hCE1) under optimized conditions. MDCK-WT and MDCK-MDR1 cell lines are utilized to identify potential modification on P-gp mediated transport of OS by herbs/herbal components. Caco-2 cells with and without Gly-Sar inhibition are performed to study the uptake of OS via PEPT1 transporters. Modification on OAT3 mediated transport is verified by the uptake of OS on HEK293-MOCK/HEK293-OAT3 cells. Anti-virus effects were evaluated using plaque reduction assay on H1N1 and H3N2 viruses. Potential pharmacokinetic and pharmacodynamic interaction between OS (30 mg/kg) and the selected herb, Radix Scutellariae (RS), at 300–600 mg/kg were carried out on rats. All samples are analyzed by an LC/MS/MS method for the contents of OS and OSA. A mechanistic PK model was developed to interpret the HDI between OS and RS in rats. RESULTS: Our developed platform was successfully applied to screen the eight herbal extracts and their ten marker components on metabolic inhibition of OS and modification of OS transport mediated by P-gp, OAT3 and PEPT1. Results from six in-vitro experiments were analyzed after converting raw data from each experiment to corresponding fold-change (FC) values, based on which Radix Scutellariae (RS) were selected to have the most HDI potential with OS. By analyzing the plasma and urine pharmacokinetic data after co-administration of OS with a standardized RS extract in rats using an integrated population pharmacokinetics model, it is suggested that RS could inhibit OS hydrolysis during absorption and increase the absorbed fraction of OS, which leads to the increased ratio of OS concentration versus that of OSA in both rat plasma and urine. Never the less, the anti-virus effects of 2.5 h post-dose rat plasma were not influenced by co-administration of OS with RS. CONCLUSION: A six-dimension in-vitro screening platform has been developed and successfully applied to find RS as a potential herb that would influence the co-administrated OS in rats. Although co-administered RS could inhibit OS hydrolysis during absorption and increase the absorbed fraction of OS, which lead to the increased ratio of OS concentration versus that of OSA in both rat plasma and urine, the anti-virus effect of OS was not influenced by co-administered RS. Elsevier B.V. 2019-10-28 2019-07-17 /pmc/articles/PMC7125811/ /pubmed/31325600 http://dx.doi.org/10.1016/j.jep.2019.112097 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhang, Yufeng
Lyu, Chunming
Fong, Sophia Yui Kau
Wang, Qian
Li, Chenrui
Ho, Nicolas James
Chan, Kay Sheung
Yan, Xiaoyu
Zuo, Zhong
Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs
title Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs
title_full Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs
title_fullStr Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs
title_full_unstemmed Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs
title_short Evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza Chinese medicinal herbs
title_sort evaluation of potential herb-drug interactions between oseltamivir and commonly used anti-influenza chinese medicinal herbs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125811/
https://www.ncbi.nlm.nih.gov/pubmed/31325600
http://dx.doi.org/10.1016/j.jep.2019.112097
work_keys_str_mv AT zhangyufeng evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT lyuchunming evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT fongsophiayuikau evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT wangqian evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT lichenrui evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT honicolasjames evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT chankaysheung evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT yanxiaoyu evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs
AT zuozhong evaluationofpotentialherbdruginteractionsbetweenoseltamivirandcommonlyusedantiinfluenzachinesemedicinalherbs