Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site()

The membrane (M) proteins of murine coronavirus (MHV) strains have been reported to contain only O-linked oligosaccharides. The predicted O-glycosylation site consisting of four amino acid residues of Ser–Ser–Thr–Thr is located immediately adjacent to the initiator Met and is well conserved among MH...

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Autores principales: Yamada, Yasuko K, Yabe, Mikiko, Ohtsuki, Takahiro, Taguchi, Fumihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 2000
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125849/
https://www.ncbi.nlm.nih.gov/pubmed/10725548
http://dx.doi.org/10.1016/S0168-1702(99)00134-3
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author Yamada, Yasuko K
Yabe, Mikiko
Ohtsuki, Takahiro
Taguchi, Fumihiro
author_facet Yamada, Yasuko K
Yabe, Mikiko
Ohtsuki, Takahiro
Taguchi, Fumihiro
author_sort Yamada, Yasuko K
collection PubMed
description The membrane (M) proteins of murine coronavirus (MHV) strains have been reported to contain only O-linked oligosaccharides. The predicted O-glycosylation site consisting of four amino acid residues of Ser–Ser–Thr–Thr is located immediately adjacent to the initiator Met and is well conserved among MHV strains investigated so far. We analyzed the nucleotide sequence of a highly virulent strain MHV-2 M-coding region and demonstrated that MHV-2 had a unique amino acid, Asn, at position 2 at the conserved O-glycosylation site. We also demonstrated that this substitution added N-linked glycans to MHV-2 M protein resulting in increment of molecular mass of MHV-2 M protein compared with JHM strain having only O-linked glycans.
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spelling pubmed-71258492020-04-08 Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site() Yamada, Yasuko K Yabe, Mikiko Ohtsuki, Takahiro Taguchi, Fumihiro Virus Res Article The membrane (M) proteins of murine coronavirus (MHV) strains have been reported to contain only O-linked oligosaccharides. The predicted O-glycosylation site consisting of four amino acid residues of Ser–Ser–Thr–Thr is located immediately adjacent to the initiator Met and is well conserved among MHV strains investigated so far. We analyzed the nucleotide sequence of a highly virulent strain MHV-2 M-coding region and demonstrated that MHV-2 had a unique amino acid, Asn, at position 2 at the conserved O-glycosylation site. We also demonstrated that this substitution added N-linked glycans to MHV-2 M protein resulting in increment of molecular mass of MHV-2 M protein compared with JHM strain having only O-linked glycans. Elsevier Science B.V. 2000-02 2000-03-16 /pmc/articles/PMC7125849/ /pubmed/10725548 http://dx.doi.org/10.1016/S0168-1702(99)00134-3 Text en Copyright © 2000 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Yamada, Yasuko K
Yabe, Mikiko
Ohtsuki, Takahiro
Taguchi, Fumihiro
Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site()
title Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site()
title_full Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site()
title_fullStr Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site()
title_full_unstemmed Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site()
title_short Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site()
title_sort unique n-linked glycosylation of murine coronavirus mhv-2 membrane protein at the conserved o-linked glycosylation site()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125849/
https://www.ncbi.nlm.nih.gov/pubmed/10725548
http://dx.doi.org/10.1016/S0168-1702(99)00134-3
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