Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study

Signaling lymphocyte activation molecule (SLAM) is one of the receptors for canine distemper virus (CDV). In this study, canine and feline cells expressing canine SLAM, designated A-72/cSLAM and CRFK/cSLAM, were established for the in vitro study of canine distemper. Recent CDV isolates, KDK-1 and 2...

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Autores principales: Nakano, Hitoshi, Kameo, Yuki, Andoh, Kiyohiko, Ohno, Yoshito, Mochizuki, Masami, Maeda, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125910/
https://www.ncbi.nlm.nih.gov/pubmed/18687538
http://dx.doi.org/10.1016/j.vetmic.2008.06.016
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author Nakano, Hitoshi
Kameo, Yuki
Andoh, Kiyohiko
Ohno, Yoshito
Mochizuki, Masami
Maeda, Ken
author_facet Nakano, Hitoshi
Kameo, Yuki
Andoh, Kiyohiko
Ohno, Yoshito
Mochizuki, Masami
Maeda, Ken
author_sort Nakano, Hitoshi
collection PubMed
description Signaling lymphocyte activation molecule (SLAM) is one of the receptors for canine distemper virus (CDV). In this study, canine and feline cells expressing canine SLAM, designated A-72/cSLAM and CRFK/cSLAM, were established for the in vitro study of canine distemper. Recent CDV isolates, KDK-1 and 246, which belong to genotypes Asia/H1 and Asia/H2, respectively, rapidly grew and produced distinct syncytia in both the SLAM-expressing cells. The virus-neutralizing (VN) test was successfully performed using these cells, and the results indicated that sera from dogs experimentally infected with KDK-1 had higher VN titers for homologous strain KDK-1 than for heterologous strain 246 and the vaccine Onderstepoort. These newly established cells expressing canine SLAM would help virological and serological analyses of canine distemper.
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spelling pubmed-71259102020-04-08 Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study Nakano, Hitoshi Kameo, Yuki Andoh, Kiyohiko Ohno, Yoshito Mochizuki, Masami Maeda, Ken Vet Microbiol Article Signaling lymphocyte activation molecule (SLAM) is one of the receptors for canine distemper virus (CDV). In this study, canine and feline cells expressing canine SLAM, designated A-72/cSLAM and CRFK/cSLAM, were established for the in vitro study of canine distemper. Recent CDV isolates, KDK-1 and 246, which belong to genotypes Asia/H1 and Asia/H2, respectively, rapidly grew and produced distinct syncytia in both the SLAM-expressing cells. The virus-neutralizing (VN) test was successfully performed using these cells, and the results indicated that sera from dogs experimentally infected with KDK-1 had higher VN titers for homologous strain KDK-1 than for heterologous strain 246 and the vaccine Onderstepoort. These newly established cells expressing canine SLAM would help virological and serological analyses of canine distemper. Elsevier B.V. 2009-01-01 2008-07-04 /pmc/articles/PMC7125910/ /pubmed/18687538 http://dx.doi.org/10.1016/j.vetmic.2008.06.016 Text en Copyright © 2008 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Nakano, Hitoshi
Kameo, Yuki
Andoh, Kiyohiko
Ohno, Yoshito
Mochizuki, Masami
Maeda, Ken
Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study
title Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study
title_full Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study
title_fullStr Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study
title_full_unstemmed Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study
title_short Establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study
title_sort establishment of canine and feline cells expressing canine signaling lymphocyte activation molecule for canine distemper virus study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125910/
https://www.ncbi.nlm.nih.gov/pubmed/18687538
http://dx.doi.org/10.1016/j.vetmic.2008.06.016
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