Reverse-transcription polymerase chain reaction/pyrosequencing to characterize neuraminidase H275 residue of influenza A 2009 H1N1 virus for rapid and specific detection of the viral oseltamivir resistance marker in a clinical laboratory

Pandemic 2009 H1N1 is normally susceptible to oseltamivir, but variants harboring the H275Y (CAC → TAC) mutation exhibit resistance. We describe the use of a combined reverse-transcription polymerase chain reaction (RT-PCR)/pyrosequencing approach to identify the H275 residue. A total of 223 specime...

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Detalles Bibliográficos
Autores principales: Bao, Jian R., Huard, Thomas K., Piscitelli, Arelis E., Tummala, Praveena R., Aleemi, Virginia E., Coon, Stephanie L., Master, Ronald N., Lewinski, Michael A., Clark, Richard B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125974/
https://www.ncbi.nlm.nih.gov/pubmed/22000086
http://dx.doi.org/10.1016/j.diagmicrobio.2011.09.003

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