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Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system

Virus‐like particles (VLPs) produced by recombinant expression of the major viral structural proteins could be an attractive method for severe acute respiratory syndrome (SARS) control. In this study, using the baculovirus system, we generated recombinant viruses that expressed S, E, M and N structu...

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Detalles Bibliográficos
Autores principales: Mortola, Eduardo, Roy, Polly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126153/
https://www.ncbi.nlm.nih.gov/pubmed/15474033
http://dx.doi.org/10.1016/j.febslet.2004.09.009
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author Mortola, Eduardo
Roy, Polly
author_facet Mortola, Eduardo
Roy, Polly
author_sort Mortola, Eduardo
collection PubMed
description Virus‐like particles (VLPs) produced by recombinant expression of the major viral structural proteins could be an attractive method for severe acute respiratory syndrome (SARS) control. In this study, using the baculovirus system, we generated recombinant viruses that expressed S, E, M and N structural proteins of SARS‐CoV either individually or simultaneously. The expression level, size and authenticity of each recombinant SARS‐CoV protein were determined. In addition, immunofluorescence and FACS analysis confirmed the cell surface expression of the S protein. Co‐infections of insect cells with two recombinant viruses demonstrated that M and E could assemble readily to form smooth surfaced VLPs. On the other hand, simultaneous high level expression of S, E and M by a single recombinant virus allowed the very efficient assembly and release of VLPs. These data demonstrate that the VLPs are morphological mimics of virion particles. The high level expression of VLPs with correct S protein conformation by a single recombinant baculovirus offers a potential candidate vaccine for SARS.
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spelling pubmed-71261532020-04-08 Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system Mortola, Eduardo Roy, Polly FEBS Lett Short Communications Virus‐like particles (VLPs) produced by recombinant expression of the major viral structural proteins could be an attractive method for severe acute respiratory syndrome (SARS) control. In this study, using the baculovirus system, we generated recombinant viruses that expressed S, E, M and N structural proteins of SARS‐CoV either individually or simultaneously. The expression level, size and authenticity of each recombinant SARS‐CoV protein were determined. In addition, immunofluorescence and FACS analysis confirmed the cell surface expression of the S protein. Co‐infections of insect cells with two recombinant viruses demonstrated that M and E could assemble readily to form smooth surfaced VLPs. On the other hand, simultaneous high level expression of S, E and M by a single recombinant virus allowed the very efficient assembly and release of VLPs. These data demonstrate that the VLPs are morphological mimics of virion particles. The high level expression of VLPs with correct S protein conformation by a single recombinant baculovirus offers a potential candidate vaccine for SARS. John Wiley and Sons Inc. 2004-10-08 2004-09-18 /pmc/articles/PMC7126153/ /pubmed/15474033 http://dx.doi.org/10.1016/j.febslet.2004.09.009 Text en FEBS Letters 576 (2004) 1873-3468 © 2015 Federation of European Biochemical Societies This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Short Communications
Mortola, Eduardo
Roy, Polly
Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system
title Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system
title_full Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system
title_fullStr Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system
title_full_unstemmed Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system
title_short Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system
title_sort efficient assembly and release of sars coronavirus‐like particles by a heterologous expression system
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126153/
https://www.ncbi.nlm.nih.gov/pubmed/15474033
http://dx.doi.org/10.1016/j.febslet.2004.09.009
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