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Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat

Specific point mutations in the human toll-like receptor 4 (TLR4) confer altered risk for diverse diseases including sepsis, aspergillosis and inflammatory bowel disease. Some of these TLR4 polymorphisms are racially specific. We hypothesised that feline TLR4 polymorphisms might underlie an observed...

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Autores principales: Whitney, J., Haase, B., Beatty, J., Barrs, V.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126157/
https://www.ncbi.nlm.nih.gov/pubmed/30885307
http://dx.doi.org/10.1016/j.vetimm.2019.02.009
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author Whitney, J.
Haase, B.
Beatty, J.
Barrs, V.R.
author_facet Whitney, J.
Haase, B.
Beatty, J.
Barrs, V.R.
author_sort Whitney, J.
collection PubMed
description Specific point mutations in the human toll-like receptor 4 (TLR4) confer altered risk for diverse diseases including sepsis, aspergillosis and inflammatory bowel disease. Some of these TLR4 polymorphisms are racially specific. We hypothesised that feline TLR4 polymorphisms might underlie an observed increased risk to infectious and inflammatory diseases in some cat breeds. The aim of this study was to identify breed-specific variations in the coding region of feline TLR4 and to model the effect of mutations on protein structure and function in silico. The entire coding region of TLR4 was sequenced in 8 groups (7 pure-bred, 1 crossbred) of domestic cats (Felis catus) comprising 158 individuals. Twenty-two single nucleotide polymorphisms (SNPs) were identified in TLR4, with 16 located in the coding region (11 non-synonymous) and four in the 3′UTR. Comparison of breed specific allelic frequencies indicated that Burmese and British shorthairs most commonly differed from other breeds. In silico analyses to predict the impact of the 11 non-synonymous variants indicated a deleterious effect on protein structure for one SNP (c.869 G > A), which was not associated with a specific breed. Overall, findings from this study do not support a role of TLR4 dysfunction in breed-predispositions to infectious diseases in domestic cats in Australia.
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spelling pubmed-71261572020-04-08 Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat Whitney, J. Haase, B. Beatty, J. Barrs, V.R. Vet Immunol Immunopathol Article Specific point mutations in the human toll-like receptor 4 (TLR4) confer altered risk for diverse diseases including sepsis, aspergillosis and inflammatory bowel disease. Some of these TLR4 polymorphisms are racially specific. We hypothesised that feline TLR4 polymorphisms might underlie an observed increased risk to infectious and inflammatory diseases in some cat breeds. The aim of this study was to identify breed-specific variations in the coding region of feline TLR4 and to model the effect of mutations on protein structure and function in silico. The entire coding region of TLR4 was sequenced in 8 groups (7 pure-bred, 1 crossbred) of domestic cats (Felis catus) comprising 158 individuals. Twenty-two single nucleotide polymorphisms (SNPs) were identified in TLR4, with 16 located in the coding region (11 non-synonymous) and four in the 3′UTR. Comparison of breed specific allelic frequencies indicated that Burmese and British shorthairs most commonly differed from other breeds. In silico analyses to predict the impact of the 11 non-synonymous variants indicated a deleterious effect on protein structure for one SNP (c.869 G > A), which was not associated with a specific breed. Overall, findings from this study do not support a role of TLR4 dysfunction in breed-predispositions to infectious diseases in domestic cats in Australia. Elsevier B.V. 2019-03 2019-02-26 /pmc/articles/PMC7126157/ /pubmed/30885307 http://dx.doi.org/10.1016/j.vetimm.2019.02.009 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Whitney, J.
Haase, B.
Beatty, J.
Barrs, V.R.
Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat
title Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat
title_full Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat
title_fullStr Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat
title_full_unstemmed Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat
title_short Breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat
title_sort breed-specific variations in the coding region of toll-like receptor 4 in the domestic cat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126157/
https://www.ncbi.nlm.nih.gov/pubmed/30885307
http://dx.doi.org/10.1016/j.vetimm.2019.02.009
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