A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle

We evaluated whether a bovine intestinal epithelial (BIE) cell line could serve as a useful in vitro model system for studying antiviral immune responses in bovine intestinal epithelial cells (IECs) and for the primary screening of immunobiotic microorganisms with antiviral protective capabilities....

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Autores principales: Chiba, Eriko, Villena, Julio, Hosoya, Shoichi, Takanashi, Naoya, Shimazu, Tomoyuki, Aso, Hisashi, Tohno, Masanori, Suda, Yoshihito, Kawai, Yasushi, Saito, Tadao, Miyazawa, Kenji, He, Fang, Kitazawa, Haruki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126301/
https://www.ncbi.nlm.nih.gov/pubmed/22047818
http://dx.doi.org/10.1016/j.rvsc.2011.10.002
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author Chiba, Eriko
Villena, Julio
Hosoya, Shoichi
Takanashi, Naoya
Shimazu, Tomoyuki
Aso, Hisashi
Tohno, Masanori
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Miyazawa, Kenji
He, Fang
Kitazawa, Haruki
author_facet Chiba, Eriko
Villena, Julio
Hosoya, Shoichi
Takanashi, Naoya
Shimazu, Tomoyuki
Aso, Hisashi
Tohno, Masanori
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Miyazawa, Kenji
He, Fang
Kitazawa, Haruki
author_sort Chiba, Eriko
collection PubMed
description We evaluated whether a bovine intestinal epithelial (BIE) cell line could serve as a useful in vitro model system for studying antiviral immune responses in bovine intestinal epithelial cells (IECs) and for the primary screening of immunobiotic microorganisms with antiviral protective capabilities. Immunofluorescent analyses revealed that toll-like receptor 3 (TLR3) was expressed in BIE cells, and the results of real-time quantitative PCR showed that these cells respond to stimulation with poly(I:C) by up-regulating pro-inflammatory cytokines and type I interferons. In addition, we demonstrated that BIE cells are useful for the primary screening of immunobiotic lactic acid bacteria strains which are able to beneficially modulate antiviral immune responses triggered by TLR3 activation in bovine IECs. The characterization of BIE cells performed in the present study represents an important step towards the establishment of a valuable bovine in vitro system that could be used for the development of immunomodulatory feed for bovine hosts.
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spelling pubmed-71263012020-04-08 A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle Chiba, Eriko Villena, Julio Hosoya, Shoichi Takanashi, Naoya Shimazu, Tomoyuki Aso, Hisashi Tohno, Masanori Suda, Yoshihito Kawai, Yasushi Saito, Tadao Miyazawa, Kenji He, Fang Kitazawa, Haruki Res Vet Sci Article We evaluated whether a bovine intestinal epithelial (BIE) cell line could serve as a useful in vitro model system for studying antiviral immune responses in bovine intestinal epithelial cells (IECs) and for the primary screening of immunobiotic microorganisms with antiviral protective capabilities. Immunofluorescent analyses revealed that toll-like receptor 3 (TLR3) was expressed in BIE cells, and the results of real-time quantitative PCR showed that these cells respond to stimulation with poly(I:C) by up-regulating pro-inflammatory cytokines and type I interferons. In addition, we demonstrated that BIE cells are useful for the primary screening of immunobiotic lactic acid bacteria strains which are able to beneficially modulate antiviral immune responses triggered by TLR3 activation in bovine IECs. The characterization of BIE cells performed in the present study represents an important step towards the establishment of a valuable bovine in vitro system that could be used for the development of immunomodulatory feed for bovine hosts. Elsevier Ltd. 2012-10 2011-11-01 /pmc/articles/PMC7126301/ /pubmed/22047818 http://dx.doi.org/10.1016/j.rvsc.2011.10.002 Text en Copyright © 2011 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Chiba, Eriko
Villena, Julio
Hosoya, Shoichi
Takanashi, Naoya
Shimazu, Tomoyuki
Aso, Hisashi
Tohno, Masanori
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Miyazawa, Kenji
He, Fang
Kitazawa, Haruki
A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
title A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
title_full A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
title_fullStr A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
title_full_unstemmed A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
title_short A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
title_sort newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126301/
https://www.ncbi.nlm.nih.gov/pubmed/22047818
http://dx.doi.org/10.1016/j.rvsc.2011.10.002
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