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Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge

Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, su...

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Autores principales: Tomar, Jasmine, Biel, Carin, de Haan, Cornelis A.M., Rottier, Peter J.M., Petrovsky, Nikolai, Frijlink, Henderik W., Huckriede, Anke, Hinrichs, Wouter L.J., Peeters, Ben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126314/
https://www.ncbi.nlm.nih.gov/pubmed/30312742
http://dx.doi.org/10.1016/j.ejpb.2018.10.008
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author Tomar, Jasmine
Biel, Carin
de Haan, Cornelis A.M.
Rottier, Peter J.M.
Petrovsky, Nikolai
Frijlink, Henderik W.
Huckriede, Anke
Hinrichs, Wouter L.J.
Peeters, Ben
author_facet Tomar, Jasmine
Biel, Carin
de Haan, Cornelis A.M.
Rottier, Peter J.M.
Petrovsky, Nikolai
Frijlink, Henderik W.
Huckriede, Anke
Hinrichs, Wouter L.J.
Peeters, Ben
author_sort Tomar, Jasmine
collection PubMed
description Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.
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spelling pubmed-71263142020-04-08 Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge Tomar, Jasmine Biel, Carin de Haan, Cornelis A.M. Rottier, Peter J.M. Petrovsky, Nikolai Frijlink, Henderik W. Huckriede, Anke Hinrichs, Wouter L.J. Peeters, Ben Eur J Pharm Biopharm Article Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings. The Authors. Published by Elsevier B.V. 2018-12 2018-10-09 /pmc/articles/PMC7126314/ /pubmed/30312742 http://dx.doi.org/10.1016/j.ejpb.2018.10.008 Text en © 2018 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Tomar, Jasmine
Biel, Carin
de Haan, Cornelis A.M.
Rottier, Peter J.M.
Petrovsky, Nikolai
Frijlink, Henderik W.
Huckriede, Anke
Hinrichs, Wouter L.J.
Peeters, Ben
Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge
title Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge
title_full Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge
title_fullStr Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge
title_full_unstemmed Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge
title_short Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge
title_sort passive inhalation of dry powder influenza vaccine formulations completely protects chickens against h5n1 lethal viral challenge
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126314/
https://www.ncbi.nlm.nih.gov/pubmed/30312742
http://dx.doi.org/10.1016/j.ejpb.2018.10.008
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