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Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment
RNA interference (RNAi) has been successfully applied in suppression of Hepatitis B virus (HBV) replication. To circumvent the problem that mutation in HBV genome may result in resistance when siRNA is further developed as an anti-viral drug, in this study, we established a dual small interfering RN...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126598/ https://www.ncbi.nlm.nih.gov/pubmed/16022904 http://dx.doi.org/10.1016/j.virusres.2005.04.001 |
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author | Wu, Kai-Lang Zhang, Xue Zhang, Jianlin Yang, Yongbo Mu, Yong-Xin Liu, Mo Lu, Lu Li, Yan Zhu, Ying Wu, Jianguo |
author_facet | Wu, Kai-Lang Zhang, Xue Zhang, Jianlin Yang, Yongbo Mu, Yong-Xin Liu, Mo Lu, Lu Li, Yan Zhu, Ying Wu, Jianguo |
author_sort | Wu, Kai-Lang |
collection | PubMed |
description | RNA interference (RNAi) has been successfully applied in suppression of Hepatitis B virus (HBV) replication. To circumvent the problem that mutation in HBV genome may result in resistance when siRNA is further developed as an anti-viral drug, in this study, we established a dual small interfering RNA (siRNA) expression system, which could simultaneously express two different siRNA molecules that can specifically target two genes. To test the effectiveness of this system, we applied this new approach to express simultaneously two different 21-bp hairpin siRNA duplexes that specifically attack the HBs and HBx genes of HBV, respectively, in Bel-7402 and HepG2.2.15 cells. Results indicated that dual siRNA could simultaneously inhibit the expression of HBs and HBx gene by 83.7% and 87.5%, respectively, based on luciferase assays. In addition, dual siRNA molecules were able to significantly reduce the amount of HBV core associated DNA, which is considered as an intracellular replicative intermediate, and the viral DNA in culture supernatant. Therefore, this dual siRNA system provides a more powerful tool for the study of gene function and implicates a potential application in the treatment of viral infection. |
format | Online Article Text |
id | pubmed-7126598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71265982020-04-08 Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment Wu, Kai-Lang Zhang, Xue Zhang, Jianlin Yang, Yongbo Mu, Yong-Xin Liu, Mo Lu, Lu Li, Yan Zhu, Ying Wu, Jianguo Virus Res Article RNA interference (RNAi) has been successfully applied in suppression of Hepatitis B virus (HBV) replication. To circumvent the problem that mutation in HBV genome may result in resistance when siRNA is further developed as an anti-viral drug, in this study, we established a dual small interfering RNA (siRNA) expression system, which could simultaneously express two different siRNA molecules that can specifically target two genes. To test the effectiveness of this system, we applied this new approach to express simultaneously two different 21-bp hairpin siRNA duplexes that specifically attack the HBs and HBx genes of HBV, respectively, in Bel-7402 and HepG2.2.15 cells. Results indicated that dual siRNA could simultaneously inhibit the expression of HBs and HBx gene by 83.7% and 87.5%, respectively, based on luciferase assays. In addition, dual siRNA molecules were able to significantly reduce the amount of HBV core associated DNA, which is considered as an intracellular replicative intermediate, and the viral DNA in culture supernatant. Therefore, this dual siRNA system provides a more powerful tool for the study of gene function and implicates a potential application in the treatment of viral infection. Elsevier B.V. 2005-09 2005-04-26 /pmc/articles/PMC7126598/ /pubmed/16022904 http://dx.doi.org/10.1016/j.virusres.2005.04.001 Text en Copyright © 2005 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Wu, Kai-Lang Zhang, Xue Zhang, Jianlin Yang, Yongbo Mu, Yong-Xin Liu, Mo Lu, Lu Li, Yan Zhu, Ying Wu, Jianguo Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment |
title | Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment |
title_full | Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment |
title_fullStr | Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment |
title_full_unstemmed | Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment |
title_short | Inhibition of Hepatitis B virus gene expression by single and dual small interfering RNA treatment |
title_sort | inhibition of hepatitis b virus gene expression by single and dual small interfering rna treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126598/ https://www.ncbi.nlm.nih.gov/pubmed/16022904 http://dx.doi.org/10.1016/j.virusres.2005.04.001 |
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