Cargando…

DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes

In this work, a gold complex is used as electroactive label for monitoring hybridization assays on glassy carbon electrodes. Ionic gold is bound to a 30-mer sequence of the SARS (severe acute respiratory syndrome) virus, responsible for the atypical pneumonia, using sodium aurothiomalate. In order t...

Descripción completa

Detalles Bibliográficos
Autores principales: de la Escosura-Muñiz, Alfredo, González-García, María Begoña, Costa-García, Agustín
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126624/
https://www.ncbi.nlm.nih.gov/pubmed/16762539
http://dx.doi.org/10.1016/j.bios.2006.04.024
_version_ 1783516186634354688
author de la Escosura-Muñiz, Alfredo
González-García, María Begoña
Costa-García, Agustín
author_facet de la Escosura-Muñiz, Alfredo
González-García, María Begoña
Costa-García, Agustín
author_sort de la Escosura-Muñiz, Alfredo
collection PubMed
description In this work, a gold complex is used as electroactive label for monitoring hybridization assays on glassy carbon electrodes. Ionic gold is bound to a 30-mer sequence of the SARS (severe acute respiratory syndrome) virus, responsible for the atypical pneumonia, using sodium aurothiomalate. In order to label this single strand, a mixture of sodium aurothiomalate and the strand is prepared. Then, it is incubated for 24 h at 37 °C and, finally, free gold is separated from the labeled strand by a dialysis against a 0.15 M NaCl solution (pH 7.5). The DNA hybridization sensor is designed immobilizing the complementary probe on the pre-treated electrode surface and, then, the hybridization reaction takes place with the gold labeled strand. The electrochemical determination is based on the catalytic effect of electrodeposited gold on the reduction of silver ions. In non-stringent experimental conditions, a limit of detection of 15 fmol (30 μL) is obtained, and discrimination between a complementary oligonucleotide and a three-based mismatch complementary oligonucleotide is achieved. For the discrimination of a single-base mismatch, is needed to use stringent conditions (50% of formamide in the hybridization buffer).
format Online
Article
Text
id pubmed-7126624
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71266242020-04-08 DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes de la Escosura-Muñiz, Alfredo González-García, María Begoña Costa-García, Agustín Biosens Bioelectron Article In this work, a gold complex is used as electroactive label for monitoring hybridization assays on glassy carbon electrodes. Ionic gold is bound to a 30-mer sequence of the SARS (severe acute respiratory syndrome) virus, responsible for the atypical pneumonia, using sodium aurothiomalate. In order to label this single strand, a mixture of sodium aurothiomalate and the strand is prepared. Then, it is incubated for 24 h at 37 °C and, finally, free gold is separated from the labeled strand by a dialysis against a 0.15 M NaCl solution (pH 7.5). The DNA hybridization sensor is designed immobilizing the complementary probe on the pre-treated electrode surface and, then, the hybridization reaction takes place with the gold labeled strand. The electrochemical determination is based on the catalytic effect of electrodeposited gold on the reduction of silver ions. In non-stringent experimental conditions, a limit of detection of 15 fmol (30 μL) is obtained, and discrimination between a complementary oligonucleotide and a three-based mismatch complementary oligonucleotide is achieved. For the discrimination of a single-base mismatch, is needed to use stringent conditions (50% of formamide in the hybridization buffer). Elsevier B.V. 2007-01-15 2006-06-09 /pmc/articles/PMC7126624/ /pubmed/16762539 http://dx.doi.org/10.1016/j.bios.2006.04.024 Text en Copyright © 2006 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
de la Escosura-Muñiz, Alfredo
González-García, María Begoña
Costa-García, Agustín
DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
title DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
title_full DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
title_fullStr DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
title_full_unstemmed DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
title_short DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
title_sort dna hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126624/
https://www.ncbi.nlm.nih.gov/pubmed/16762539
http://dx.doi.org/10.1016/j.bios.2006.04.024
work_keys_str_mv AT delaescosuramunizalfredo dnahybridizationsensorbasedonaurothiomalateelectroactivelabelonglassycarbonelectrodes
AT gonzalezgarciamariabegona dnahybridizationsensorbasedonaurothiomalateelectroactivelabelonglassycarbonelectrodes
AT costagarciaagustin dnahybridizationsensorbasedonaurothiomalateelectroactivelabelonglassycarbonelectrodes