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Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina

The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7 %) healthy and 225 (36.3 %) diarrheic calves, belonging to 45 dairy farms in Co...

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Autores principales: Picco, Natalia Y., Alustiza, Fabrisio E., Bellingeri, Romina V., Grosso, María C., Motta, Carlos E., Larriestra, Alejandro J., Vissio, Claudina, Tiranti, Karina I., Terzolo, Horacio R., Moreira, Ana R., Vivas, Adriana B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asociación Argentina de Microbiología. Published by Elsevier España S.L. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126665/
https://www.ncbi.nlm.nih.gov/pubmed/26026231
http://dx.doi.org/10.1016/j.ram.2015.01.006
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author Picco, Natalia Y.
Alustiza, Fabrisio E.
Bellingeri, Romina V.
Grosso, María C.
Motta, Carlos E.
Larriestra, Alejandro J.
Vissio, Claudina
Tiranti, Karina I.
Terzolo, Horacio R.
Moreira, Ana R.
Vivas, Adriana B.
author_facet Picco, Natalia Y.
Alustiza, Fabrisio E.
Bellingeri, Romina V.
Grosso, María C.
Motta, Carlos E.
Larriestra, Alejandro J.
Vissio, Claudina
Tiranti, Karina I.
Terzolo, Horacio R.
Moreira, Ana R.
Vivas, Adriana B.
author_sort Picco, Natalia Y.
collection PubMed
description The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7 %) healthy and 225 (36.3 %) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1 % isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.
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spelling pubmed-71266652020-04-08 Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina Picco, Natalia Y. Alustiza, Fabrisio E. Bellingeri, Romina V. Grosso, María C. Motta, Carlos E. Larriestra, Alejandro J. Vissio, Claudina Tiranti, Karina I. Terzolo, Horacio R. Moreira, Ana R. Vivas, Adriana B. Rev Argent Microbiol Article The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7 %) healthy and 225 (36.3 %) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1 % isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli. Asociación Argentina de Microbiología. Published by Elsevier España S.L. 2015 2015-05-27 /pmc/articles/PMC7126665/ /pubmed/26026231 http://dx.doi.org/10.1016/j.ram.2015.01.006 Text en Copyright © 2014 Asociación Argentina de Microbiología. Published by Elsevier España S.L. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Picco, Natalia Y.
Alustiza, Fabrisio E.
Bellingeri, Romina V.
Grosso, María C.
Motta, Carlos E.
Larriestra, Alejandro J.
Vissio, Claudina
Tiranti, Karina I.
Terzolo, Horacio R.
Moreira, Ana R.
Vivas, Adriana B.
Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_full Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_fullStr Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_full_unstemmed Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_short Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina
title_sort molecular screening of pathogenic escherichia coli strains isolated from dairy neonatal calves in cordoba province, argentina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126665/
https://www.ncbi.nlm.nih.gov/pubmed/26026231
http://dx.doi.org/10.1016/j.ram.2015.01.006
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