Cargando…
RNA silencing of rotavirus gene expression
RNA interference (RNAi) is a double-stranded RNA (dsRNA)-triggered mechanism for suppressing gene expression, which is conserved in evolution and has emerged as a powerful tool to study gene function. Rotaviruses, the leading cause of severe diarrhea in young children, are formed by three concentric...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2004
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126860/ https://www.ncbi.nlm.nih.gov/pubmed/15068879 http://dx.doi.org/10.1016/j.virusres.2004.01.014 |
_version_ | 1783516235691982848 |
---|---|
author | Arias, Carlos F Dector, Miguel A Segovia, Lorenzo López, Tomás Camacho, Minerva Isa, Pavel Espinosa, Rafaela López, Susana |
author_facet | Arias, Carlos F Dector, Miguel A Segovia, Lorenzo López, Tomás Camacho, Minerva Isa, Pavel Espinosa, Rafaela López, Susana |
author_sort | Arias, Carlos F |
collection | PubMed |
description | RNA interference (RNAi) is a double-stranded RNA (dsRNA)-triggered mechanism for suppressing gene expression, which is conserved in evolution and has emerged as a powerful tool to study gene function. Rotaviruses, the leading cause of severe diarrhea in young children, are formed by three concentric layers of protein, and a genome composed of 11 segments of dsRNA. Here, we show that the RNAi machinery can be triggered to silence rotavirus gene expression by sequence-specific short interfering RNAs (siRNAs). RNAi is also useful for the study of the virus-cell interactions, through the silencing of cellular genes that are potentially important for the replication of the virus. Interestingly, while the translation of mRNAs is readily stopped by the RNAi machinery, the viral transcripts involved in virus genome replication do not seem to be susceptible to RNAi. Since gene silencing by RNAi is very efficient and specific, this system could become a novel therapeutic approach for rotavirus and other virus infections, once efficient methods for in vivo delivery of siRNAs are developed. Although the use of RNAi as an antiviral therapeutic tool remains to be demonstrated, there is no doubt that this technology will influence drastically the way postgenomic virus research is conducted. |
format | Online Article Text |
id | pubmed-7126860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71268602020-04-08 RNA silencing of rotavirus gene expression Arias, Carlos F Dector, Miguel A Segovia, Lorenzo López, Tomás Camacho, Minerva Isa, Pavel Espinosa, Rafaela López, Susana Virus Res Article RNA interference (RNAi) is a double-stranded RNA (dsRNA)-triggered mechanism for suppressing gene expression, which is conserved in evolution and has emerged as a powerful tool to study gene function. Rotaviruses, the leading cause of severe diarrhea in young children, are formed by three concentric layers of protein, and a genome composed of 11 segments of dsRNA. Here, we show that the RNAi machinery can be triggered to silence rotavirus gene expression by sequence-specific short interfering RNAs (siRNAs). RNAi is also useful for the study of the virus-cell interactions, through the silencing of cellular genes that are potentially important for the replication of the virus. Interestingly, while the translation of mRNAs is readily stopped by the RNAi machinery, the viral transcripts involved in virus genome replication do not seem to be susceptible to RNAi. Since gene silencing by RNAi is very efficient and specific, this system could become a novel therapeutic approach for rotavirus and other virus infections, once efficient methods for in vivo delivery of siRNAs are developed. Although the use of RNAi as an antiviral therapeutic tool remains to be demonstrated, there is no doubt that this technology will influence drastically the way postgenomic virus research is conducted. Elsevier B.V. 2004-06-01 2004-03-02 /pmc/articles/PMC7126860/ /pubmed/15068879 http://dx.doi.org/10.1016/j.virusres.2004.01.014 Text en Copyright © 2004 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Arias, Carlos F Dector, Miguel A Segovia, Lorenzo López, Tomás Camacho, Minerva Isa, Pavel Espinosa, Rafaela López, Susana RNA silencing of rotavirus gene expression |
title | RNA silencing of rotavirus gene expression |
title_full | RNA silencing of rotavirus gene expression |
title_fullStr | RNA silencing of rotavirus gene expression |
title_full_unstemmed | RNA silencing of rotavirus gene expression |
title_short | RNA silencing of rotavirus gene expression |
title_sort | rna silencing of rotavirus gene expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126860/ https://www.ncbi.nlm.nih.gov/pubmed/15068879 http://dx.doi.org/10.1016/j.virusres.2004.01.014 |
work_keys_str_mv | AT ariascarlosf rnasilencingofrotavirusgeneexpression AT dectormiguela rnasilencingofrotavirusgeneexpression AT segovialorenzo rnasilencingofrotavirusgeneexpression AT lopeztomas rnasilencingofrotavirusgeneexpression AT camachominerva rnasilencingofrotavirusgeneexpression AT isapavel rnasilencingofrotavirusgeneexpression AT espinosarafaela rnasilencingofrotavirusgeneexpression AT lopezsusana rnasilencingofrotavirusgeneexpression |